Ir al menú de navegación principal Ir al contenido principal Ir al pie de página del sitio

Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration

Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration



Abrir | Descargar

Cómo citar
Díaz T, P., Bernal G, A., & López C, C. (2014). Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration. Revista MVZ Córdoba, 19(3), 4338-4349. https://doi.org/10.21897/rmvz.95

Dimensions
PlumX
Paula Díaz T
Adriana Bernal G
Camilo López C

ABSTRACT

Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β-glucuronidase (GUS). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves.


Visitas del artículo 886 | Visitas PDF


Descargas

Los datos de descarga todavía no están disponibles.
  1. Olsen KM, SNPs, SSRs and inferences on cassava's origin. Plant Mol Biol 2004; 56(4):517-26. http://dx.doi.org/10.1007/s11103-004-5043-9
  2. Taylor NJ, Fauquet CM, Tohme J. Overview of Cassava Special Issue. Tropical Plant Biol 2012; 5:1-3. http://dx.doi.org/10.1007/s12042-012-9098-5
  3. FAO. Cassava's huge potential as 21st Century crop. [en línea]. Roma: Organización de las Naciones Unidades para la Agricultura y la Alimentación. 2013. [consultado 30 Mayo 2013]. URL disponible en: http://www.fao.org/news/story/en/item/176780/icode/
  4. Ceballos H, Okogbenin E, Pérez JC, López-Valle LAB, Debouck D. Cassava. In: Bradshaw JE, editor. Root and tuber crops. London: SpringerLink; 2010. http://dx.doi.org/10.1007/978-0-387-92765-7_2
  5. Balat M, Balat H. Recent trends in global production and utilization of bio-ethanol fuel. Applied Energy 2009; 86(11):2273-82. http://dx.doi.org/10.1016/j.apenergy.2009.03.015
  6. El-Sharkawy MA. Cassava biology and physiology. Plant mol biol 2004; 56(4):481-501. http://dx.doi.org/10.1007/s11103-005-2270-7
  7. Von Bubnoff A. Next-Generation Sequencing: The Race Is On. Cell 2008; 132(5):721-3. http://dx.doi.org/10.1016/j.cell.2008.02.028
  8. Yamada K, Lim J, Dale JM, Chen H, Shinn P, Palm CJ, et al. Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome. Science 2003; 302(5646):842-846. http://dx.doi.org/10.1126/science.1088305
  9. Prochnik S, Marri PR, Desany B, Rabinowicz PD, Kodira C, Mohiuddin M, et al. The Cassava Genome: Current Progress, Future Directions. Tropical Plant Biol 2012; 5:88-94.
  10. http://dx.doi.org/10.1007/s12042-011-9088-z
  11. Wroblewski T, Tomczak A, Michelmore R. Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol J 2005; 3(2):259-73. http://dx.doi.org/10.1111/j.1467-7652.2005.00123.x
  12. Voinnet O, Rivas S, Mestre P, Baulcombe D. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 2003; 33:949-56. http://dx.doi.org/10.1046/j.1365-313X.2003.01676.x
  13. Kohli A, Twyman RM, Abranches R, Wegel E, Stoger E, Christou P. Transgene integration, organization and interaction in plants. Plant Mol Biol 2003; 52(2):247-258. http://dx.doi.org/10.1023/A:1023941407376
  14. Ma L, Lukasik E, Gawehns F, Takken FLW. The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves. Cap: Bolton MD, Thomma BPHJ, editors. Plant Fungal Pathogens: Methods and Protocols. Amsterdam: SpringerLink; 2012. http://dx.doi.org/10.1007/978-1-61779-501-5_4
  15. McCullen CA, Binns AN. Agrobacterium tumefaciens and Plant Cell Interactions and Activities Required for Interkingdom Macromolecular Transfer. Annu Rev Cell Dev Biol 2006; 22(1):101-27. http://dx.doi.org/10.1146/annurev.cellbio.22.011105.102022
  16. Lee M, Yang Y. Transient Expression Assay by Agroinfiltration of Leaves. In: Salinas J, Sanchez-Serrano JJ, editors. Arabidopsis protocols. New Jersey: Humana Press Inc; 2006. http://dx.doi.org/10.1385/1-59745-003-0:225
  17. Dodds PN, Lawrence GJ, Catanzariti AM, Teh T, Wang CI, Ayliffe MA, et al. Direct protein interaction underlies gene-for-gene specificity and coevolution of the flax resistance genes and flax rust avirulence genes. Proc Natl Acad Sci USA 2006; 103(23):8888-93. http://dx.doi.org/10.1073/pnas.0602577103
  18. Leckie BM, Neal Stewart C. Agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants. Plant cell rep 2010; 30(3):325-34. http://dx.doi.org/10.1007/s00299-010-0961-2
  19. Taylor N, Chavarriaga P, Raemakers K, Siritunga D, Zhang P. Development and application of transgenic technologies in cassava. Plant mol biol 2004; 56(4):671-88. http://dx.doi.org/10.1007/s11103-004-4872-x
  20. Bull SE, Owiti JA, Niklaus M, Beeching JR, Gruissem W, Vanderschuren H. Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava. Nat Protoc 2009; 4(2):1845-54. http://dx.doi.org/10.1038/nprot.2009.208
  21. Sayre R, Beeching JR, Cahoon EB, Egesi C, Fauquet C, Fellman J, et al. The BioCassava Plus Program: Biofortification of Cassava for Sub-Saharan Africa. Annu Rev Plant Biol 2011; 62(1):251-72. http://dx.doi.org/10.1146/annurev-arplant-042110-103751
  22. Alzate A M, Vallejo F, Ceballos H, Pérez J C, Fregene M. Variabilidad genética de la yuca cultivada por peque-os agricultores de la región Caribe de Colombia. Acta agronómica 2010; 59(10):385-393.
  23. Beltrán J, Prías M, Al-Babili S, Ladino Y, López D, Beyer P, et al. Expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (Manihot esculenta Crantz). Planta 2010; 231(6):1413-24. http://dx.doi.org/10.1007/s00425-010-1144-7
  24. Kim MJ, Baek K, Park C-M. Optimization of conditions for transient Agrobacterium-mediated gene expression assays in Arabidopsis. Plant cell Rep 2009; 28(8):1159-67. http://dx.doi.org/10.1007/s00299-009-0717-z
  25. Simmons CW, VanderGheynst JS, Upadhyaya SK. A model of Agrobacterium tumefaciens vacuum infiltration into harvested leaf tissue and subsequent in planta transgene transient expression. Biotechnol Bioeng 2009; 102(3):965-70. http://dx.doi.org/10.1002/bit.22118

Sistema OJS 3.4.0.3 - Metabiblioteca |