Using of essential oils in the treatment of mice infected with Trypanosoma evansi

Contenido principal del artículo

Autores

Matheus D Baldissera Aleksandro S Da Silva Camila B Oliveira Rodrigo A Vaucher Roberto CV Santos Janice L Giongo Dianni M Capeleto Alexandre A Tonin Silvia G Monteiro

Resumen

ABSTRACT

Objective. This study aimed to test the effectiveness of copaiba, andiroba and aroeira essential oils for controlling trypanosomosis by Trypanosoma evansi with mice as experimental model. Materials and methods. Sixty-six mice were divided into eleven groups (A to L) with six animals each. Group A was the unique composed by healthy and uninfected animals (negative control). Animals in groups B to L were inoculated with 0.1 mL of blood containing 2.7 x 106 trypanosomes. Group B was used as a positive control without treatment. In experiment were tested copaiba (C, D and E), andiroba (F, G and H) and aroeira (I, J and L) oils at doses of 0.6, 0.8 and 1.0 mL kg-1 to infected mice (T. evansi). Results. These protocols did not provide curative efficacy; however, the mice treated with highest dose of copaiba showed a significant increase in the longevity when compared others groups. Conclusions. Previously in our studies, these essential oils have shown trypanocidal activity in vitro, but when they were tested in vivo in mice infected with T. evansi, this trypanocidal activity, or the curative effect was not found, being only able to prolong the lifespan of the animals treated with copaiba oil.

Palabras clave:

Detalles del artículo

Referencias

1. Pinto AC, Silva DHS, Bolzani VS, Lopes NP, Epifanio RA. Produtos naturais: atualidade, desafios e perspectivas. Químíca Nova 2002; 25:45-61. http://dx.doi.org/10.1590/S0100-40422002000800009

2. Maciel MAM, Pinto AC, Júnior VF. Plantas medicinais: A necessidade de estudos interdisciplinares. Química Nova 2002; 25: 429–438. http://dx.doi.org/10.1590/S0100-40422002000300016

3. Amorim MMR, Santos LC. Tratamento da vaginose bacteriana com gel vaginal de Aroeira (Schinus terebinthifolius Raddi): ensaio clínico randomizado. Rev Bras Ginecol Obstet 2003; 25:95-102. http://dx.doi.org/10.1590/S0100-72032003000200004

4. Marongiu B, Alessandra PSP, Casu R, Pierucci P. Chemical composition of the oil and supercritical CO2 extracts of Schinus molle L. Flav Frag J 2004; 19:554-558. http://dx.doi.org/10.1002/ffj.1350

5. Araújo Jr FA, Braz MN, Neto OGR, Costa FD, Brito MVH. Efeito do óleo de copaíba nas aminotransferases de ratos submetidos à isquemia e reperfusão hepática com e sem pré-condicionamento isquêmico. Acta Cir Bras 2005; 20:93-99. http://dx.doi.org/10.1590/s0102-86502005000100013

6. Veiga VF, Patitucci ML, Pinto AC. Authenticity control of commercial copaiba oils by high resolution gas chromatography. Química Nova 1997; 20:612-615.

7. Paiva LAF, Gurgel LA, Silva RM, Tomé AR, Gramosa NV, Silveira ER, Santos FA, Rao VNS. Anti-inflammatory effect of kaurenoic acid, a diterpene from Copaifera langsdorffi on acetic acid-induced colitis in rats. Vascular Pharmacol 2002; 39:303-307. http://dx.doi.org/10.1016/S1537-1891(03)00028-4

8. Oliveira ECP de, Lameira OA, Barros PLC, Poltronieri LS. Avaliação do óleo de copaíba (Copaifera) na inibição do crescimento micelial In vitro de fitopatógenos. Rev Ciênc Agr 2006; 46:53-61.

9. Farias MPO, Sousa DP, Arruda AC, Arruda MSP, Wanderley AG, Alves LC, Faustino MAG. Eficácia In vitro do óleo da Carapa guianensis Aubl. (Andiroba) no controle de Boophilus microplus (Acari: Ixodidae). Rev Bras Plan Med 2007; 9:68-71.

10. Gilbert B, Teixeira DF, Carvalho ES, De Paula AE, Pereira JF, Almeida MB, Machado RS, Cascon V. Activities of the pharmaceutical technology Institute of the Oswaldo Cruz Fundation with medicinal, insecticidal and repellent plants. Anais Acad Bras Ciênc 1999; 71:265–271.

11. Moura MD, Souza – Silva J, De Oliveira RAG, Diniz MFFM, Barbosa – Filho JM. Natural products reported as potencial inibitors of uterine cervical neoplasia. Acta Farmac Bonar 2002; 21:67–74.

12. Penido C, Costa KA, Costa MF, Pereira JF, Siani AC, Henriques MG. Inhibition of allergen – induced eosinophil recruitment by natural tetranortriterpenoids is mediated by the suppression of IL-5, CCL11/eotaxin and NfkappaB activation. Int Immunopharmacol 2006; 6:109-121. http://dx.doi.org/10.1016/j.intimp.2005.07.011

13. Santos RBC, Dos Santos Alves CF, Schneider T, Lopes LQ, Aurich C, Giongo JL, Brandelli A, Vaucher RA. Antimicrobial activity of Amazonian oils against Paenibacillus species. J Inve Pathol 2012; 109:265-268. http://dx.doi.org/10.1016/j.jip.2011.12.002

14. Mendonça FA, Silva KF, Santos KK, Ribeiro Júnior KA, Sant'ana AE. Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti. Fitorerapia 2005; 76:629-636. http://dx.doi.org/10.1016/j.fitote.2005.06.013

15. Izumi E. Efeitos do óleo de copaíba e constituintes em Trypanosoma cruzi, 95f. Londrina, PR. [Tese Doutorado em Microbiologia] – Programa de Pós- graduação em Microbiologia, Universidade Federal de Londrina, 2010.

16. Baldissera MD, Da Silva AS, Oliveira CB, Zimmermann CEP, Vaucher RA, Santos RCV, Rech VC, Tonin AA, Giongo JL, Mattos CB, Koester L, Santurio JM, Monteiro SG. Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: In vitro tests. Exp Parasitol 2013; 134:156-161. http://dx.doi.org/10.1016/j.exppara.2013.03.035

17. Silva RAMS, Seidl A, Ramirez L, Dávila AMR. Trypanosoma evansi e Trypanosoma vivax: biologia, diagnóstico e controle. Corumbá, Brasil: Embrapa Pantanal; 2002.

18. Maudlin I, Holmes P, Miles MA. The trypanosomiases. Wallingford, Inglaterra: CABI Publishing; 2004. http://dx.doi.org/10.1079/9780851994758.0000

19. Peregrine AS, Mamman M. Pharmacology of Diminazene: A Review. Acta Tropica 1993; 54:185–203. http://dx.doi.org/10.1016/0001-706X(93)90092-P

20. Colpo CB, Colpo ETB, Stainki DR, Monteiro SG. Infecção Natural por Trypanosoma evansi em cão no Rio Grande do Sul. Ciência Rural 2005; 35:717-719. http://dx.doi.org/10.1590/S0103-84782005000300038

21. Santos AO, Ueda-Nakamura T, Dias Filho BP, Veiga Junior VFV, Lima MMS, Nakamura CV. Leishmania amazonensis: Effects of oral treatment with copaiba oil in mice. Exp Parasitol 2011; 129:154-151. http://dx.doi.org/10.1016/j.exppara.2011.06.016

22. Dornelles F, Da Silva AS, Oliveira CB, Dalla-Rosa L, Zimmermann CEP, Tonin AA, Oliveira ECP, Santurio, JM, Monteiro SG. Susceptibility of Trypanosoma evansi in the copaiba oil: In vitro test and in mice experimentally infected with the parasite. Acta Scie Vet 2013; 41:1136.

23. Izumi E, Ueda – Kakamura T, Veiga Júnior VF, Pinto AC, Nakamura CV. Terpenes from Copaifera demonstrated In vitro antiparasitic and synergic activity. J Med Chem 2012; 55:2994–3001. http://dx.doi.org/10.1021/jm201451h

Descargas

La descarga de datos todavía no está disponible.