Evaluación de implantes de norgestomet reutilizados en protocolos de sincronización del estro en vacas Brahman

Contenido principal del artículo

Autores

Luis Uribe-Velásquez Adriana Correa-Orozco Lina Cuartas-Betancurth Diego Villamizar-Ramirez Santiago Ángel-Botero

Resumen

RESUMEN

Objetivo. Evaluar las concentraciones de progesterona, la manifestación de estro y las tasas de preñez en vacas Bos indicus sometidas a varios protocolos de sincronización del estro con un implante de norgestomet usado previamente. Materiales y métodos. Sesenta vacas recibieron un implante auricular de norgestomet reutilizado y fueron distribuidas en uno de cuatro protocolos: (1) benzoato de estradiol (BE) + progesterona (P4) + prostaglandina F2α (PG) (BE+P4+PG); (2) valerato de estradiol (VE) + norgestomet (NG) (VE+NG); (3) el mismo protocolo BE+P4+PG, asociado con 400 UI de gonadotropina coriónica equina (eCG) (BE+P4+PG+eCG); (4) el mismo protocolo VE+NG, asociado con 400 UI de eCG (VE+NG+eCG). El implante fue removido el día 9, con inseminación artificial (IA) 12 horas después de la detección del estro. La preñez fue diagnosticada 45 días después de la IA. Las muestras de sangre fueron tomadas los días 0, 4 y 9 (después de colocar el implante) para el análisis de progesterona por RIA. Resultados. En el día 4, las concentraciones de progesterona fueron menores en vacas tratadas con BE+P4+PG (0.90 ± 0.73 ng/ml; p<0.01). Las vacas que recibieron VE+NG+eCG tuvieron mayor concentración de progesterona en plasma (p<0.05) en el día 9 (4.72 ± 0.79 ng/mL) que otros protocolos. Hubo mayor porcentaje de detección de estro (p<0.05) en vacas tratadas con VE+NG+eCG (66.7%). Las tasas de preñez no fueron afectadas por los protocolos (p>0.05). Conclusiones. Los implantes de norgestomet reutilizados fueron eficaces para sincronizar el estro y alcanzar tasas de preñez adecuadas en vacas Brahman.

Palabras clave:

Detalles del artículo

Referencias

1. Sá Filho MF, Ayres H, Ferreira RM, Marques MO, Reis EL, Silva RCP et al. Equine chorionic gonadotropin and gonadotropinreleasing hormone enhance fertility in a norgestomet-based, timed artificial insemination protocol in suckled Nelore (Bos indicus) cows. Theriogenology 2010a; 73:651-658.

2. Báez G, Grajales H. Anestro posparto en ganado bovino en el trópico. Rev MVZ Córdoba 2009; 14(3):1867-1875.

3. Meneghetti M, Sá Filho OG, Peres RFG, Lamb GC, Vasconcelos JLM. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: Basis for development of protocols. Theriogenology 2009; 72:179–189. http://dx.doi.org/10.1016/j.theriogenology.2009.02.010

4. Sá Filho MF, Crespilho AM, Santos JEP, Perry GA, Baruselli OS. Ovarian follicle diameter at timed insemination and estrous response influence likelihood of ovulation and pregnancy after estrous synchronization with progesterone or progestin-based protocols in suckled Bos indicus cows. Anim Reprod Sci 2010b; 120:23–30.

5. Lamb GC, Dahlen CR, Larson JE, Marquezini G, Stevenson JS. Control of the estrous cycle to improve fertility for fixed-time artificial insemination in beef cattle: a review. J Anim Sci 2010; 88:E181-E192.

6. Mihm M. Delayed resumption of cyclicity in postpartum dairy and beef cows. Reprod Dom Anim 1999; 34:277-284. http://dx.doi.org/10.1111/j.1439-0531.1999.tb01252.x

7. Peres RFG, Claro Júnior I, Sá Filho OG, Nogueira GP, Vasconcelos JLM. Strategies to improve fertility in Bos indicus postpubertal heifers and nonlactating cows submitted to fixed-time artificial insemination. Theriogenology 2009; 72:681–689. http://dx.doi.org/10.1016/j.theriogenology.2009.04.026

8. Dias CC, Wechsler FS, Day ML, Vasconcelos JLM. P roge s te rone con cen t ra tion s , exogenous equine chorionic gonadotropin, and timing of prostaglandin F2α treatment affect fertility in postpuberal Nelore heifers. Theriogenology 2009; 72:378-385. http://dx.doi.org/10.1016/j.theriogenology.2009.03.006

9. Almeida AB, Bertan CM, Rossa LAF, Gaspar PS, Binelli M, Madureira EH. Avaliação da reutilização de implantes auriculares contendo norgestomet associados ao valerato ou ao benzoato de estradiol em vacas Nelore inseminadas em tempo fixo. Braz J Vet Res Anim Sci 2006; 43(4):456-465.

10. Maluf DZ, Pires AV, Susin I, Moreira RJC, Madureira EH, Binelli M. Avaliação da reutilização de implantes contendo progestágenos na taxa de prenhez em vacas de corte. Braz J Vet Res Anim Sci 2010; 47(1):38-46.

11. Colazo MG, Kastelic JP, Whittaker PR, Gavaga QA, Wilde R, Mapletoft RJ. Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Anim Reprod Sci 2004; 81:25-34. http://dx.doi.org/10.1016/j.anireprosci.2003.09.003

12. Solórzano CF, Mendoza JH, Galina C, Villa A, Vera HR, Romo S. Reutilización de un dispositivo liberador de progesterona (CIDR-B) para sincronizar el estro en un programa de transferencia de embriones bovinos. Téc Pecu Méx 2008; 46(2):119-135.

13. Richards M, Spitzer J, Warner M. Effect of varying levels of postpartum nutrition and body condition at calving on subsequent reproductive performance in beef cattle. J Anim Sci 1986; 62:300-306. http://dx.doi.org/10.2527/jas1986.622300x

14. SAS Institute Inc.®: Guide for Personal computer [programa de ordenador]. Versión 6.12. Cary (NC): SAS Institute IC.; 1985.

15. Sartori R, Bastos MR, Baruselli PS, Gimenes LU, Ereno RL, Barros CM. Phisiologycal differencies and implications to reproductive management of Bos taurus and Bos indicus cattle in a tropical environment. Soc Reprod Fertil Suppl 2010; 67:357-375.

16. Sá Filho OG, Dias CC, Lamb GC, Vasconcelos JLM. P r o g e s t e r o n e - b a s e d e s t r o u s synchronization protocols in non-suckled and suckled primiparous Bos indicus beef cows. Anim Reprod Sci 2010; 119:9–16. http://dx.doi.org/10.1016/j.anireprosci.2009.12.011

17. Pinheiro OL, Barros CM, Figueiredo RA, Do Valle ER, Encarnação RO, Padovani CR. Estrous behavior and the estrus-toovulation interval in Nelore cattle (Bos indicus) with natural estrus or estrus induced with prostaglandin F2α or norgestomet and estradiol valerate. Theriogenology 1998; 49:667-681. http://dx.doi.org/10.1016/S0093-691X(98)00017-X

18. Galina CS, Orihuela A. The detection of estrus in cattle raised under tropical conditions: What we know and what we need to know. Horm Behav 2007; 52:32-38. http://dx.doi.org/10.1016/j.yhbeh.2007.03.025

19. Solano J, Orihuela A, Galina CS, Montiel F. Sexual behavior of Zebu cattle (Bos indicus) following estrous induction by Syncro-Mate B, with or without estrogen injection. Physiol Behav 2000; 71:503-508. http://dx.doi.org/10.1016/S0031-9384(00)00367-X

20. Kastelic JP, Olson WO, Martinez M, Cook RB, Mapletoft RJ. Synchronization of estrus in beef cattle with norgestomet and estradiol valerate. Can Vet J 1999; 40:173-178.

21. Cavalieri J, Rubio I, Kinder JE, Entwistle KW, Fitzpatrick LA. Synchronization of estrus and ovulation and associated endocrine changes in Bos indicus cows. Theriogenology 1997; 47:801-814. http://dx.doi.org/10.1016/S0093-691X(97)00036-8

22. Duffy P, Crowe MA, Austin EJ, Mihm M, Boland MP, Roche JF. The effect of eCG or estradiol at or after norgestomet removal on follicular dynamics, estrus and ovulation in early post-partum beef cows nursing calves. Theriogenology 2004; 61:725-734. http://dx.doi.org/10.1016/S0093-691X(03)00255-3

23. Murphy BD, Martinuk SD. Equine chorionic gonadotropin. Endocr Rev 1991;12:1305-1319.

24. Pegorer MF, Ereno RL, Satrapa RA, Pinheiro VG, Trinca LA, Barros CM. Neither plasma progesterone concentrations nor exogenous eCG affects rates of ovulation or pregnancy in fixed-time artificial insemination (FTAI) protocols for puberal Nellore heifers. Theriogenology 2011; 75:17-23. http://dx.doi.org/10.1016/j.theriogenology.2010.07.005

25. Pinheiro VG, Souza AF, Pegorer MF, Satrapa RA, Ereno RL, Trinca LA et al. Effects of temporary calf removal and eCG on pregnancy rates to timed-insemination in progesterone-treated postpartum Nellore cows. Theriogenology 2009; 71:519-524. http://dx.doi.org/10.1016/j.theriogenology.2008.08.018

26. Sá Filho OG, Meneghetti M, Peres RFG, Lamb GC, Vasconcelos JLM. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting fertility. Theriogenology 2009; 72:210–218. http://dx.doi.org/10.1016/j.theriogenology.2009.02.008

27. Sá Filho MF, Torres-Júnior JRS, Penteado L, Gimenes LU, Ferreira RM, Ayres H et al. Equine chorionic gonadotropin improves the efficacy of a progestin-based fixed-time artificial insemination protocol in Nelore (Bos indicus) heifers. Anim Reprod Sci 2010c; 118:182–187.

28. Rossa LAF, Bertan CM, Almeida AB, Gaspar PS, Mazza PH, Binelli M et al. Efeito do eCG ou benzoato de estradiol associado ao norgestomet na taxa de concepção de vacas de corte submetidas à IATF no pós-parto. Braz. J Vet Res Anim Sci 2009; 46(3):199-206.

29. Baruselli PS, Reis EL, Marques MO, Nasser LF, Bo GA. The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Anim Reprod Sci 2004; 83:479-486. http://dx.doi.org/10.1016/j.anireprosci.2004.04.025

Descargas

La descarga de datos todavía no está disponible.