Efecto de Debaryomyces hansenii en la respuesta antioxidante de juveniles de camarón blanco Litopenaeus vannamei

Contenido principal del artículo

Autores

María Pacheco M Ángel Campa C Gabriel Aguirre G Antonio Luna G María Guzmán M Felipe Ascencio

Resumen

RESUMEN

Objetivo. Determinar la respuesta antioxidante [actividad de superóxido dismutasa (SOD) y catalasa (CAT)] así como la cuenta total de hemocitos (CTH) y el contenido de proteínas (CP) en camarones (Litopenaeus vannamei) expuestos a diferentes dosis y cepas de la levadura Debaryomyces hansenii (DH5, DH6, LL1), y un inmunoestimulante comercial (LAM). Materiales y métodos. Las levaduras fueron cultivadas y suministradas diariamente en concentraciones diferentes (104 – 106 UFC/mL) directamente a los tanques de cultivo de los camarones (8 ± 0.2 g) mientras que LAM fue aplicado una vez a la semana (0.5 mg/L). Los organismos fueron mantenidos bajo condiciones de laboratorio (28°C, 35%, 80% de recambio diario de agua, dieta comercial para camarón ad libitum). Los tratamientos fueron distribuidos por duplicado y los resultados evaluados a los 15 días con un análisis de varianza y una prueba de Tukey. Resultados. Se registró un CTH significativo (p<0.05) en los tratamientos con DH6 y LL1 (106 UFC/mL) comparada con el control, mientras que las cepas DH5 y DH6 revelaron un incremento significativo (p<0.05) de CP con la dosis de 104 UFC/mL. Los camarones tratados con LAM incrementaron significativamente (p<0.05) los valores de SOD y CAT. Conclusiones. Los resultados obtenidos demuestran que D. hansenii incrementa la respuesta antioxidante y CTH en camarones.

Palabras clave:

Detalles del artículo

Referencias

1. Aguirre-Guzmán G, Sánchez-Martínez JG, Campa-Córdova AI, Luna-González A, Ascencio F. Penaeid shrimp immune system: A Minireview. Thai J Vet Med 2009; 39: 205-215.

2. Zhen-Ming C, Liu G, Zhao S, Li J, Peng Y. Marine yeasts as biocontrol agents and producers of bio-products. Appl Microbiol Biotechnol 2010; 86:1227-1241. http://dx.doi.org/10.1007/s00253-010-2483-9

3. Vici V, Bright Sing IS, Bhat SG. Application of bacterins and yeast Acremonium dyosporii to protect the larvae of Macrobrachium rosenbergii from vibriosis. Fish Shellfish Immunol 2000; 10:559-563. http://dx.doi.org/10.1006/fsim.2000.0278

4. Robles R, Sorgeloos P, Van Duffel H, Nelis H. Progress in biomedication using live foods. J Appl Ichthyol 1998; 14:207-212. http://dx.doi.org/10.1111/j.1439-0426.1998.tb00643.x

5. Miles DJC, Polchana J, Lilley JH, Kanchanakhan S, Thompson KD, Adams A. Immunostimulation of striped snakehead Channa striata against epizootic ulcerative syndrome. Aquaculture 2001; 195:1-15. http://dx.doi.org/10.1016/S0044-8486(00)00529-9

6. Gatesoupe FJ. Live yeasts in the gut: natural occurrence, dietary introduction, and their effects on fish health and development. Aquaculture 2007; 267:20-30. http://dx.doi.org/10.1016/j.aquaculture.2007.01.005

7. Reyes-Becerril M, Salinas I, Cuesta A, Meseguer J, Tovar-Ramirez D, AscencioValle F, Esteban MA. Oral delivery of live yeast Debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol 2008a; 25:731-739.

8. Reyes-Becerril M, Tovar-Ramirez D, AscencioValle F, Civera-Cerecedo R, Gracia-López V, Barbosa-Solomieu V. Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress. Aquaculture 2008b; 280: 39–44.

9. Sukumaran V, Williams DL, Sajeevan TP, Rosamma P. Marine yeast glucans confer better protection than that of baker's yeast in Penaeus monodon against white spot syndrome virus infection. Aquac Res 2010; 41: 1799-1805. http://dx.doi.org/10.1111/j.1365-2109.2010.02520.x

10. Mu-oz M, Cedeno R, Rodriguez J, Knaap WPW, Mialhe E, Bachere E. Measurement of reactive oxygen intermediate production in haemocytes of penaeid shrimp, Penaeus vannamei. Aquaculture 2000; 191: 89-107. http://dx.doi.org/10.1016/S0044-8486(00)00420-8

11. Campa-Córdova AI, Hernández-Saavedra NY, Philippis R De, Ascencio F. Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to β-glucan and sulphated polysaccharide. Fish Shellfish Immunol 2002; 12: 353-366. http://dx.doi.org/10.1006/fsim.2001.0377

12. Silakes S, Supamattaya K. Immunostimulant and vaccination in Black Tiger shrimp, Penaeus monodon Fabricius: II Production of vaccine from Vibrio harveyi and its application in Black Tiger shrimp, Penaeus monodon Fabricius. Songklanakarin J Sci Technol 2000; 22(Suppl.): 663-676.

13. Sajeevan TP, Lowman DW, Williams DL, Selven S, Anas A, Rosamma P. Marine yeast diet confers better protection than its cell wall component (1-3)-b-D-glucan as an immunostimulant in Fenneropenaeus indicus. Aquac Res 2009; 40: 1723-1730. http://dx.doi.org/10.1111/j.1365-2109.2009.02275.x

14. Yang SP, Wu ZH, Jian JC, Zhang XZ. Effect of marine red yeast Rhodosporium paludigenum on growth and antioxidant competence of Litopenaeus vannamei. Aquaculture 2010; 309: 62-65. http://dx.doi.org/10.1016/j.aquaculture.2010.09.032

15. Tovar D, Zambonino-Infante J, Cahu C, Gatesoupe FJ, Vázquez-Juárez R. Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development. Aquaculture 2004; 234: 415–427. 16. Shupantharika M, Khunrae P, Thanardkit P, Verduyn C. Preparation of spent brewer's yeast β-glucans with potential application as an immunostimulant for black tiger shrimp, Penaeus monodon. Bioresource Technol 2003; 88: 55-60. 17. Macey BM, Coyne VE. Improved growth rate and disease resistance in farmed Haliotis midae through probiotic treatment. Aquaculture 2005; 245: 249-261. http://dx.doi.org/10.1016/j.aquaculture.2004.11.031

18. Vine NG, Leukes WD, Horst K. Probiotics in marine larviculture. FEMS Microbiol Rev 2006; 30: 404-427. http://dx.doi.org/10.1111/j.1574-6976.2006.00017.x

19. Boonyaratpalin M, Supamattaya K, Verakunpiriya V, Suprasert D. Effects of aflatoxin B1 on growth performance, blood components, immune function and histopathological changes in black tiger shrimp (Penaeus monodon Fabricius). Aquac Res 2001; 32: 388-398. http://dx.doi.org/10.1046/j.1355-557x.2001.00046.x

20. Sritunyalucksana K, Gangnonngiw W, Archakunakorn S, Fegan D, Flegel TW. Bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. Dis Aquat Org 2005; 63: 89–94. http://dx.doi.org/10.3354/dao063089

21. Laria RE, Cruz Y, Silveira R, Martínez M, González N. Effects of formalin on total haemocytes count and histopathological changes in the shrimp Litopenaeus schmitti (Pérez-Farfante & Kensley 1997). Aquac Res 2008; 39: 1316-1321. http://dx.doi.org/10.1111/j.1365-2109.2008.01997.x

22. Sajeevan TP, Selven S, Rosamma P. b-Mercapto-ethanol-treated yeast showed better protection against white spot syndrome virus infection in Indian white shrimp Fenneropenaeus indicus. 2010. Aquac Res 2010; 41: e715-e718.

23. Downs C, Fauth JE, Woodley CM. Assessing the health of grass shrimp (Palaemonetes pugio) exposed to natural and anthropogenic stressors: A molecular biomarker system. Mar Biotechnol 2001; 3: 380-397. http://dx.doi.org/10.1007/s10126-001-0008-3

24. Moreno I, Pichardo S, Jose A, GómezAmores L, Mate A, Vázquez CM, Camean AM. Antioxidant enzyme activity and lipid peroxidation in liver and kidney of rats exposed to microcystin-LR administered intraperitoneally. Toxicon 2005; 45:395-402. http://dx.doi.org/10.1016/j.toxicon.2004.11.001

25. Van de Braak K. Hemocytic defense in black tiger shrimp (Penaeus monodon). [Doctor degree thesis]. The Netherlands: Wageningen University. Wageningen Institute of Animal Science; 2002.

26. Liu CH, Yeh SP, Kuo CH, Cheng W, Chou CH. The effect of sodium alginate on the immune response of tiger shrimp via dietary administration: Activity and gene transcription. Fish Shellfish Immunol 2006; 21:442-452. http://dx.doi.org/10.1016/j.fsi.2006.02.003

27. Guertler C, Schleder DD, Barracco MA, Perazzolo LM. Comparative study of the intracellular superoxide anion production in different penaeid species through the NBT-reduction assay. Aquac Res 2010; 41: 1082-1088.

Descargas

La descarga de datos todavía no está disponible.