Prevalence of Listeria monocytogenes in pork-meat and other processed products from the Colombian swine industry

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Andrea Gamboa-Marín Sonia Buitrago M Karol Pérez-Pérez Marcela Mercado R Raúl Poutou-Piñales Ana Carrascal-Camacho

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ABSTRACT

Objective. To determine the prevalence of L. monocytogenes in pork carcasses, meat cuts, and meat products (“chorizo”, sausage and ham). Materials and methods. Stratified sampling was implemented in meat-processed products. We analyzed 566 (37%) carcasses, 472 (31%) meat cuts, and 481, (32%) meat-processed products, distributed as follows: 169 (11%) sausage, 163 (11%) ham, and 149 (10%) “chorizo”, for a total of 1519 (100%) samples in a period of 18 months. The samples were processed using the ISO-17604, ISO-11290-1 and the USDA/FSIS (MLG-8.03) methods. Genus and species were confirmed by multiplex-PCR. Results. We obtained isolates of L. monocytogenes from 21 carcasses (10%), 160 (76%) from meat deboning, 10 (5%) from ham, 6 (3%) from “chorizo”, and 13 (6%) from sausage. The prevalence found was 3.7% and 33.9% in carcasses and meat deboning respectively. The prevalence in the meat-processed products was 4.03% in “chorizo”, 6.13% in ham and 7.69% in sausage. The overall prevalence of L. monocytogenes in the study was 13.82%. Conclusions. We found L. monocytogenes in different products analyzed, with particular interest in ham and sausage since both are consumed without previous heat treatment

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Referencias

1. Torres KJ, Sierra SC, Poutou RA, Vera H, Carrascal AK, Mercado M. Incidencia y diagnóstico de Listeria monocytogenes; microorganismo zoonótico emergente en la industria de alimentos. Rev UDCA Act Divulg Cient 2004; 7(1):25-57.

2. Torres KJ, Sierra SC, Poutou RA, Carrascal AK, Mercado M. Patogénesis de Listeria monocytogenes, microorganismo zoonó- tico emergente. Rev MVZ Córdoba 2005; 10(1):511-543.

3. Belalcazar ME, Poutou RA, Torres KJ, Gallegos JM, Torres O, Carrascal AK. Listeria monocytogenes y listeriosis animal. Rev UDCA Act Inv Cient 2005; 8(2):95-101.

4. Fox EM, Leonard N, Jordan K. Molecular diversity of Listeria monocytogenes isolated from Irish dairy farms. FPD 2011; 8(5):635-641.

5. Vanegas MC, Vasquez E, Martinez AJ, Rueda AM. Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR. Food Cont 2009; 20:430-432. http://dx.doi.org/10.1016/j.foodcont.2008.07.007

6. Vaillant V, De Valk H, Baron E, Ancelle T, Colin P, Delmas B et al. Foodborne infections in France. FPD 2005; 2(3):221-232. http://dx.doi.org/10.1089/fpd.2005.2.221

7. Taillefer C, Boucher M, Laferrière C, Morin L. Perinatal listeriosis: Canada's 2008 Outbreaks. J Obst Gynaec Canada 2010; 32(1):45-48. http://dx.doi.org/10.1016/S1701-2163(16)34403-6

8. Bohaychuk VM, Gensler GE, King RK, Manninen KI, Sorensen O, Wu JT et al. Occurrence of pathogens in raw and ready-to-eat meat and poultry products collected from the retail marketplace in Edmonton, Alberta, Canada. J Food Prot 2006; 69(9):2176-2182.

9. Mu-oz AI, Vargas M, Otero L, Díaz G, Guzmán V. Presencia de Listeria monocytogenes en alimentos listos para el consumo, procedentes de plazas de mercado y delicatessen de supermercados de cadena en Bogotá D.C, 2002-2008. Biomédica 2011; 31(3):428-439.

10. Consejo Nacional de Política Económica y Social (Conpes 3458). Política nacional de sanidad e inocuidad para la cadena porcícola In. Bogotá. D.C. Colombia: Ministerio de Agricultura y Desarrollo Rural, Ministerio de Protección Social, Ministerio de Ambiente, Vivienda y Desarrollo Territorial, Ministerio de Comercio, Industria y Turismo; 2007.

11. Vera H, Ferro CJ, Triana LM. Prevalencia de Listeria moncytogenes en derivados cárnicos cocidos para consumo directo analizados en el laboratorio de salud pública, Bogotá 1 de septiembre 2001- 31 agosto de 2004. In. Bogotá, D.C., Colombia: Laboratorio de Salud Pública, Secretaría Distrital de Salud, Bogotá, D.C., Colombia; 2006.

12. ISO. International Standardizartion Organization: Microbiology of food and animal feeding stuffs-carcass sampling for microbiological analysis (ISO17604) 2003.

13. USDA-FSIS. Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, Egg and Environmental Samples. MLG 8.07. In: USDA/FSIS Microbiology Laboratory Guidebook. Washington, D.C.: United States Department of Agriculture. Food Safety and Inspection Service; 2002.

14. Meza RA, Monroy AF, Mercado M, Poutou RA, Rodríguez P, Pedroza AM. Study of the stability in real time of cryopreserved strain banks. Univ Sci 2004; 9(2):35-42.

15. Sambrook J, Russell DW. Molecular cloning: A laboratory manual, vol. 3, 3th Edition edn. Appendices. New York: Cold Spring Harbor Laboratory Press; 2001.

16. Ruiz-Bolivar Z, Carrascal-Camacho AK, Neuque-Rico MC, Gutiérrez-Trivi-o C, Rodríguez-Bocanegra MX, Poutou-Pi-ales RA et al. Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) fingerprinting reveals intra-serotype variations among circulating Listeria monocytogenes strains. Afr J Microbiol Res 2011; 5(13):1586-1598.

17. Hernández-Chavarría F. Fundamentos de epidemiología: el arte detectivesco de la investigación epidemiológica. Costa Rica: EUNED; 2002.

18. Molero G, Tarradas C, Ramírez I, Gallardo F, Montiel M. Aislamiento e identificación de Listeria monocytogenes durante el proceso de beneficio de pollos en plantas beneficiadoras en el estado Zulia, Venezuela. Ciencia 2010; 18(2):108-114.

19. Martins EA, Leal Germano PM. Listeria monocytogenes in ready-to-eat, sliced, cooked ham and salami products, marketed in the city of São Paulo, Brazil: Occurrence, quantification, and serotyping. Food Cont 2011; 22(2):297-302. http://dx.doi.org/10.1016/j.foodcont.2010.07.026

20. Ochiai Y, Yamada F, Batmunkh O, Mochizuki M, Takano T, Hondo R et al. Prevalence of Listeria monocytogenes in retailed meat in the Tokyo metropolitan area. J Food Prot 2010; 73(9):1688-1693.

21. Hellstrom S, Laukkanen R, Siekkinen KM, Ranta J, Maijala R, Korkeala H. Listeria monocytogenes contamination in pork can originate from farms. J Food Prot 2010; 73(4):641-648.

22. Kanuganti SR, Wesley IV, Reddy PG, McKean J, Hurd HS. Detection of Listeria monocytogenes in Pigs and Pork. J Food Prot 2002; 65(9):1470-1474.

23. Nightingale KK, Fortes ED, Ho AJ, Schukken YH, Grohn YT, Wiedmann M. Evaluation of farm management practices as risk factors for clinical listeriosis and fecal shedding of Listeria monocytogenes in ruminants. J Amer Vet Med Associat 2005; 227(11):1808-1811. http://dx.doi.org/10.2460/javma.2005.227.1808

24. Peccio A, Autio T, Korkeala H, Rosmini R, Trevisani M. Listeria monocytogenes occurrence and characterization in meatproducing plants. Lett Appl Microbiol 2003; 37(3):234-238. http://dx.doi.org/10.1046/j.1472-765X.2003.01384.x

25. Jessen B, Lammert L. Biofilm and disinfection in meat processing plants. Int Biodet Biod 2003; 51(4):265-269. http://dx.doi.org/10.1016/S0964-8305(03)00046-5

26. Chasseignaux E, Gérault P, Toquin MT, Salvat G, Colin P, Ermel G. Ecology of Listeria monocytogenes in the Environment of Raw Poultry Meat and Raw Pork Meat Processing Plants. FEMS Microbiol Let 2002; 210:271-275. http://dx.doi.org/10.1111/j.1574-6968.2002.tb11192.x

27. Lundén JM, Autio TJ, Korkeala HJ. Transfer of persistent Listeria monocytogenes contamination between food processing plants associated with a dicing machine. J Food Prot 2002; 7:1129-1133.

28. Thévenot D, Dernburg A, VernozyRozand C. An updated review of Listeria monocytogenes in the pork meat industry and its products. J Appl Microbiol 2006; 101:7-17. http://dx.doi.org/10.1111/j.1365-2672.2006.02962.x

29. Wesley IV, Larsen S, Hurd HS, McKean JD, Griffith R, Rivera F et al. Low prevalence of Listeria monocytogenes in cull sows and pork. J Food Prot 2008; 71(3):545-549.

30. Karakolev R. Incidence of Listeria monocytogenes in beef, pork, raw-dried and raw-smoked sausages in Bulgaria. Food Cont 2009; 20:953-955. http://dx.doi.org/10.1016/j.foodcont.2009.02.013

31. Miyasaki KN, Chiarini E, Sant Ana AD, Destro MT, Landgraf M, Franco BD. High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguica, a Brazilian fresh pork sausage. Meat Sci 2009; 83:523-527. http://dx.doi.org/10.1016/j.meatsci.2009.06.037

32. De Cesare A, Mioni R, Manfreda G. Prevalence of Listeria monocytogenes in fresh and fermented Italian sausages and ribotyping of contaminating strains. Int J Food Microbiol 2007; 120(1-2):124-130.

33. Cabedo L, Picart i Barrot L, Teixido i Canelles A. Prevalence of Listeria monocytogenes and Salmonella in readyto-eat food in Catalonia, Spain. J Food Prot 2008; 71(4):855-859.

34. Chasseignaux E, Toquin MT, Ragimbeau C, Salvat G, Colin P, Ermel G. Molecular epidemiology of Listeria monocytogenes isolates collected from the environment, raw meat and raw products in two poultry and pork processing plants. J Appl Microbiol 2001; 91(5):888-899. http://dx.doi.org/10.1046/j.1365-2672.2001.01445.x

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