Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

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Autores

Rosa Sanmiguel P Iang Rondón B

Resumen

ABSTRACT

Objective. Asses the effect of supplementation with Humic substances (HS) over some innate immunity parameters (serum bactericidal activity, phagocytosis, bacterial agglutination, respiratory burst and lisozyme activity) in phase after fasting of layer hens. Materials and methods. 120 posfasting phase Hy Line Brown layer hens were taken which were distributed into four groups: The first and the second were supplemented with 0.1 and 0.2% of HS, respectively. The third group was supplemented with 0.25 mg/kg on levamisole hydrochloride and fourth group have no supplementation; during sixty days period. Blood samples were collected on 8th, 30th and 60th of experiment day. Results. The phagocytic index and respiratory burst increased significantly at day 30th in HS supplemented groups. Alike, serum bactericidal activity and lisozyme activity improved on 8 th day, nevertheless, changes were no evident latter. The bacterial agglutination was high in supplemented groups evaluated at everyone times. Conclusions. Results showed that HS behave as immunostimulant in the early phase after fasting layer hens.

RESUMEN

Objetivo. Evaluar el efecto de las sustancias húmicas (SH) sobre algunos parámetros de la inmunidad innata (actividad bactericida del suero, fagocitosis, aglutinación bacteriana, explosión respiratoria y actividad de la lisozima) en la fase posmuda de gallinas ponedoras. Materiales y métodos. Se utilizaron 120 gallinas ponedoras Hy Line Brown en la fase de posmuda, las cuales fueron divididas en cuatro grupos: Los dos primeros fueron suplementados con 0.1 y 0.2% de SH respectivamente, el tercer grupo fue suplementado con 0.25 mg/kg de Clorhidrato de levamisol y el cuarto grupo control sin suplemento; durante un período de 60 días. Las muestras sanguíneas se tomaron los días 8, 30 y 60 del experimento. Resultados. El Índice fagocítico y la explosión respiratoria se incrementaron significativamente a partir del día 30 de suplementación con SH. De la misma manera, la actividad bactericida del suero y la actividad de la lisozima aumentaron al día 8; no obstante no se evidenciaron cambios posteriores. La aglutinación bacteriana fue significativamente mayor en los grupos suplementados en todos los tiempos evaluados. Conclusiones. Los resultados demuestran que las SH se comportan como agentes inmunoestimulantes en la fase temprana de la posmuda en gallinas ponedoras Hy Line Brown.

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Referencias

1. Van den A, London N, Driessen C, Stobberingh E. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and slaughterers. J Antimicrob Chemoter 2001; 47(6):763-771. http://dx.doi.org/10.1093/jac/47.6.763

2. Pomorska-Mol L, Pejsak Z. Effects of antibiotics on acquired immunity in vivo current state of knowledge. Pol J Vet Sci 2012; 15(3):583-588.

3. Akhtar M, Tariq A, Awais M, Iqbal Z, Muhammad F, Shahid M, Hiszczynska-Sawicka E. Studies on wheat bran arabinoxylan for its immunoestimulatory and protective effects avian coccidiosis. Carbohydr Polym 2012; 90(1):333-339. http://dx.doi.org/10.1016/j.carbpol.2012.05.048

4. Foster N, Berndt A, Lalmanach A, Methner U, Pascquali P, Rychlich I, et al Emergency and therapeutic vaccination - is stimulating innate immunity an option?. Res Vet Sci 2012; 93(1):7-12. http://dx.doi.org/10.1016/j.rvsc.2011.05.018

5. Wondmeneh E, Getachew T., Dessie T. Effect of Effective microorganisms (EM®) on the growth parameters of fayoumi and Horro chicken. Int J Poult Sci 2011; 10(3):185-188. http://dx.doi.org/10.3923/ijps.2011.185.188

6. Vlcová Z. Chemical and physical transformations of humic acids. [Tesis Doctoral]. BRNO University id technology, Faculty of chemistry, Institute of physical and applied chemistry: Brno, República Checa; 2009.

7. Ergin O, Ocak N, Turan A, Erener G, Altop A, Cankaya S. Performance, carcass, gastrointestinal tract and meat quality traits and selected blood parameters of broilers fed diets supplemented with humic substances. J Sci Food Agric 2011; 92(1):59-65.

8. Eren M, Gezen S, Deniz G, Orhan F. Effects of liquid humate supplemented to drinking water on the performance and eggshell quality of hens in different laying periods. Revue Med Vet 2008; 159(2):91-95.

9. Sanmiguel R, Rondon I. Suplementación con sustancias húmicas en gallinas ponedoras durante la fase posmuda. Rev CES Med Zootec. 2014; 9(2): 169-178.

10. El-Deek A & Al-harthi M. Post molt performance parameters of broiler breeder hens associated with molt induced by feed restriction, high dietary zinc and fasting. Int J Poult Sci 2004; 3(7):456-462. http://dx.doi.org/10.3923/ijps.2004.456.462

11. Hassanien H. Effect of force molting programs on egg production and quality of laying hens. Asian J Poult Sci 2011; 5(1):13-20. http://dx.doi.org/10.3923/ajpsaj.2011.13.20

12. Tega E, Ibrahim T. Effect of induced molting on fertility and hatchability chickens. Continental J Anim Vet Res 2010; 2(1):31-34.

13. Moreno-Rueda G. Experimental test of a trade of between moult and immune response in house sparrows Passer domesticus. J Evol Biol 2010; 23(10):2229-2237. http://dx.doi.org/10.1111/j.1420-9101.2010.02090.x

14. Akram M, Zia-ur-rahman C, Kim S. Effect on the induced molting on the relative weights and hormone levels of thyroid, ovary and adrenal glands in spent laying hens. Korean J Poult Sci 2002; 29(4):243-247.

15. Millet S, Bennett J, Lee K, Hau M, Klasing K. Quantifying and comparing constitutive across avian species. Dev Comp Immunol 2007; 31(2):188-201. http://dx.doi.org/10.1016/j.dci.2006.05.013

16. Wells L, Lowry V, Deloach J, Kogut M. Age dependent phagocytosis and bactericidal activities of the chicken heterophil. Dev Comp Immunol 1998; 22(1):103-109. http://dx.doi.org/10.1016/S0145-305X(97)00024-4

17. Mohanty B, Sahoo P. Immune response and expression profiles of some imune related genes in Indian major carp, Labeo rohita to Edwardsiella tarda infection. Fish Shellfish Immunol 2010; 28(4):613-621. http://dx.doi.org/10.1016/j.fsi.2009.12.025

18. Behera T, Nanda T, Mohanty C, Mohapatra D, Swain P, Das B, et al. Parenteral immunization of fish, Labeo rohita whit poly D L- lactide Co- glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses. Fish Shellfish Immunol 2010; 28(2):320-325. http://dx.doi.org/10.1016/j.fsi.2009.11.009

19. Low K, Sin P. In vivo and in vitro effects of mercuric chloride and sodium selenite on some non-specific immune responses of blue gourami, Thricogaster trichopterus (Pallus). Fish Shellfish Immunol 1996; 6(5):351-362. http://dx.doi.org/10.1006/fsim.1996.0035

20. Harmon B. Avian heterophils in inflammation and disease resistance. Poult Sci 1998; 77: 972-977. http://dx.doi.org/10.1093/ps/77.7.972

21. Lim TS, Na K, Choi EM, Chung JY, Hwang JK, Immunomodulating activities of polysaccharides isolated from Panax ginseng. J Med Food 2004; 7(1):1–6. http://dx.doi.org/10.1089/109662004322984626

22. Engstad C, Engstad R, Olsen J, Osterud B. The effect of soluble -1,3-glucan and lipopolysaccharide on cytokine production and coagulation activation in whole blood. Int J Immunopharmacol 2002; 2(11):1585–1597. http://dx.doi.org/10.1016/S1567-5769(02)00134-0

23. Huammitri P, Redmond S, Kimura K, Andreasen C, Lamont S, Palic D. Heterophil functional responses to dietary immunomodulators vary in genetically distinct chicken lines. Vet immunol immunopathol 2011; 142(3):219-227. http://dx.doi.org/10.1016/j.vetimm.2011.05.019

24. Jiao l, Wank D, Zhang X, Li B, Zhao H, Liu S. Characterization and immunostimulating effects on murine peritoneal of oligosaccharide isolated from Panax ginseng C.A Meyer. J Ethnopharmacol 2012; 144(3):490-496. http://dx.doi.org/10.1016/j.jep.2012.09.004

25. Swaggerty C, Pevzner I, Ferro P, Crippen T, Kogut M. Association between in vitro heterophil function and the feathering gene in commercial broiler chickens. Avian Pathol 2003; 32(5):433-438. http://dx.doi.org/10.1080/0307945031000154071

26. Li B, Y Huang, Paskewitz S. Hen egg white lysozyme as an inhibitor of mushroom tyrosinase. FEBS Letter 2006; 580(7):1877-1882. http://dx.doi.org/10.1016/j.febslet.2006.02.051

28. Babu U, Gaines D, Lillehoj H, Rayburn R. Differential reactive oxygen and nitrogen production and clearance of Salmonella serovars by chicken and mouse macrophages. Dev comp immunol 2006; 30(10);942-953. http://dx.doi.org/10.1016/j.dci.2005.12.001

29. Woodward C, Kwon Y, Kubena N, Byrd J, Moore R, Nisbet D, Ricke S. Reduction of Salmonella enterica serovar enteritidis colonization and invasion by an alfalfa diet during molt in leghorn hens. Poult Sci 2005; 84(2):185-193. http://dx.doi.org/10.1093/ps/84.2.185

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