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Efecto de gonadotropinas sobre la maduración y desarrollo embrionario de oocitos bovinos cultivados in vitro

Efecto de gonadotropinas sobre la maduración y desarrollo embrionario de oocitos bovinos cultivados in vitro



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Salgado O, R., Vergara G, O., & Ramirez P, L. (2010). Efecto de gonadotropinas sobre la maduración y desarrollo embrionario de oocitos bovinos cultivados in vitro. Revista MVZ Córdoba, 15(1). https://doi.org/10.21897/rmvz.333

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PlumX
Roger Salgado O
Oscar Vergara G
Leonardo Ramirez P

Objetivo. Evaluar el efecto de las hormonas FSH y LH sobre las tasas de maduración, fertilización y desarrollo embrionario in vitro. Materiales y métodos. Se utilizaron 1309 oocitos de ovarios de matadero, donde el medio base fue TCM-199 suplementado (FSH y LH) y se conformaron cuatro tratamientos al azar: T1: sin hormonas, T2: 50 µg/ml FSH + 150 µg/ml LH; T3: 100 µg/ml FSH + 100 µg/ml LH y T4: 150 µg/ml FSH + 50 µg/ml LH. Resultados. No se presentó efecto (p>0.05) de los tratamientos suplementados con FSH y LH sobre la tasa de maduración; por el contrario, T1 fue diferente (p0.05) cuando el medio de maduración fue suplementado con FSH y LH. Conclusiones. La adición de LH en mayor proporción con respecto a la FSH en el medio de maduración induce una mayor tasa de maduración, fecundación y desarrollo embrionario in vitro a partir de oocitos bovinos recuperados postmortem.

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  1. Dielman SJ, Hendriksen PJ, Viuff D, Thomsen PD, Hyttel P, Knijn HM, et al. Effect of in vivo prematuration and in vivo final maturation on developmental capacity and quality of pre-implantation embryos. Theriogenology 2002; 57:5-20. http://dx.doi.org/10.1016/S0093-691X(01)00655-0
  2. Rizos D, Ward F, Duffy P, Boland MP, Lonergan P. Consequences of bovine oocyte maturation, fertilization or early embryo development In vitro versus in vivo: implications for blastocyst yeld and blastocyst quality. Mol Reprod Dev 2002; 61:234-248. http://dx.doi.org/10.1002/mrd.1153
  3. Li YH, Jiao LH, Liu RH, Chen XL, Wang H, Wang WH. Localization of angiotensin II in pig ovary and its effects on oocyte maturation In vitro. Theriogenology 2004; 61:447-459. http://dx.doi.org/10.1016/S0093-691X(03)00246-2
  4. Kito S, Bavister BD. Male pronuclear formation and early embryonic development of hamster oocytes matured In vitro with gonadotrophin, amino acids and cysteamine. J Repro Fertil 1997; 110:35-46. http://dx.doi.org/10.1530/jrf.0.1100035
  5. Downs SM, Mastropolo AM. Culture conditions affect meiotic regulation in cumulus cell-enclosed mouse oocyte. Mol Reprod Dev 1997; 46:551-566. http://dx.doi.org/10.1002/(SICI)1098-2795(199704)46:4<551::AID-MRD13>3.0.CO;2-Z http://dx.doi.org/10.1002/(SICI)1098-2795(199704)46:4<551::AID-MRD13>3.3.CO;2-5
  6. Moor RM, Trouson AO. Hormonal and follicular factors affecting maturation of sheep In vitro and their subsequent developmental capacity. J Reprod Fertil 1997; 49:101-109.
  7. Zuelke KA, Brackett, BG. Luteinizing hormones enhanced In vitro maturation of bovine oocyte with and without protein supplementation. Biol Reprod 1990; 43:784-787. http://dx.doi.org/10.1095/biolreprod43.5.784
  8. Saeki K, Hoshi M, Leibfried-Rutledge ML, First NL. In vitro fertilization and development of bovine oocytes matured in serum-free medium. Biol Reprod 1991; 44:256-260. http://dx.doi.org/10.1095/biolreprod44.2.256
  9. Sirad MA, Desrosier S, Assidi M. In vivo and In vitro effects of FSH on oocyte maturation and developmental competence. Theriogenology 2007; 68(Supl 1): S71-S76.
  10. Eppig JJ, Viveiros MM, Bivens CM, De La Fuente R. The Ovary (Second Edition). En: Regulation of mammalian oocyte maturation. Part II. [En linea]. USA: ACADEMIC PRESS; 2003. [fecha de acceso 16 de octubre de 2008]. URL disponible en: http://www.science-direct.com/science/book/9780124445628.
  11. Mehlmann LM, Kalinowski RR, Ross LF, Parlow AF, Hewlett EL, Jaffe LA. Meiotic resumption in response to luteinizing hormone is independent of a Gi family G protein or calcium in the mouse oocyte. Dev Biol 2006; 299:345-355. http://dx.doi.org/10.1016/j.ydbio.2006.07.039
  12. Bilodeau S, Fortier MA, Sirard MA. Effect of adenylate ciclasa stimulation on meiotic resumption and cyclic AMP content on zone-free and cumulus-enclosed bovine oocyte In vitro. J Reprod Fertil 1993; 97:5-11. http://dx.doi.org/10.1530/jrf.0.0970005
  13. Plancha CE, Albertini DF. Hormonal regulation of meiotic maturation in the hamster oocyte involves a cytoskeleton-mediated process. Biol Reprod 1994; 51:852-864. http://dx.doi.org/10.1095/biolreprod51.5.852
  14. Instituto geográfico Agustín Codazzi, Córdoba. Características geográficas. Santa Fe de Bogotá: 1995.
  15. Ratto M, Berland M, Wolter M. et al. Desarrollo de embriones de bovino obtenidos por fecundación In vitro cultivados con células oviductales o medio condicionado y transferidos a hembras receptoras. Arch Med vet 1999; 31(1):89-96. http://dx.doi.org/10.4067/S0301-732X1999000100009
  16. Chohan KR, Hunter AG. In vitro maturation, fertilization and early cleavage rates of bovine fetal oocytes. Theriogenology 2004; 61:373-380. http://dx.doi.org/10.1016/S0093-691X(03)00220-6
  17. Boelhauve M, Sinowatz F, Wolf E and Paula-Lopes F. Maturation of bovine oocytes in the presence of leptin improves development and reduces apoptosis of In vitro produced blastocysts. Biol Reprod 2005;73:737–744. http://dx.doi.org/10.1095/biolreprod.105.041103
  18. Bevers MM, Dieleman SJ, Van den Hurk R, Izadyar F. Regulation and modulation of oocyte maturation in the bovine. Theriogenology 1997; 47:13-22. http://dx.doi.org/10.1016/S0093-691X(96)00335-4
  19. Sirard MA. Resumption of meiosis: mechanism involved in meiotic progression and its relation with developmental competence. Theriogenology 2001; 55:1241-1254. http://dx.doi.org/10.1016/S0093-691X(01)00480-0
  20. Conti M, Bo Andersen C, Richard F, Mehats C, Chun S, Horner K, et al. Role of cyclic nucleotide signaling in oocyte maturation. Mol Cell Endocrinol 2002; 187:153-159. http://dx.doi.org/10.1016/S0303-7207(01)00686-4
  21. Sirard MA, Richard F, Mayes M. Controlling meiotic resumption in bovine oocytes: a review. Thenogenology 1996; 49:483-497. http://dx.doi.org/10.1016/S0093-691X(97)00420-2
  22. Chian RC, Niwa K, Sirard MA. Effects of cumulus cell on male pronuclear formation and subsecuente early development of bovine oocytes In vitro. Theriogenology 1994; 41:1499-1508. http://dx.doi.org/10.1016/0093-691X(94)90201-S
  23. Luvoni GC, Chigioni S, Perego L, Lodde V, Modina S, Luciano AM. Effect of gonadotropins during In vitro maturation of feline oocyte-cúmulos cell functional coupling and intracellular concentration of glutathione. Anim Reprod Sci 2006; 96:66-78. http://dx.doi.org/10.1016/j.anireprosci.2005.11.009
  24. Bauskin AR, Franken DR, Eberspaecher U, Donner P. Characterization of human zona pellucida glycoproteins. Mol Hum Reprod 1999; 5(6):534-540. http://dx.doi.org/10.1093/molehr/5.6.534
  25. Bleil JD, Wassarman PM. Sperm-egg interactions in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein. Dev Biol 1983; 95(2):317-24. http://dx.doi.org/10.1016/0012-1606(83)90032-5
  26. Florman HM, Wassarman PM. O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity. Cell 1985; 41(1):313-24. http://dx.doi.org/10.1016/0092-8674(85)90084-4
  27. Choi YH, Carnevale EM, Steidel GE, Squires J, Squires EL. Effect of gonadotrophin on bovine oocitos matured in TCM-199. Theriogenology 2001; 56:61-670. http://dx.doi.org/10.1016/S0093-691X(01)00597-0
  28. Younis AI, Brackett BG, Fayer-Hosken RA. Influence of serum and hormones on bovine oocyte maturation and fertilization In vitro. Gamete Res 1989; 23:189-201. http://dx.doi.org/10.1002/mrd.1120230206

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