Efecto de gonadotropinas sobre la maduración y desarrollo embrionario de oocitos bovinos cultivados in vitro

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Roger Salgado O Oscar Vergara G Leonardo Ramirez P

Resumen

Objetivo. Evaluar el efecto de las hormonas FSH y LH sobre las tasas de maduración, fertilización y desarrollo embrionario in vitro. Materiales y métodos. Se utilizaron 1309 oocitos de ovarios de matadero, donde el medio base fue TCM-199 suplementado (FSH y LH) y se conformaron cuatro tratamientos al azar: T1: sin hormonas, T2: 50 µg/ml FSH + 150 µg/ml LH; T3: 100 µg/ml FSH + 100 µg/ml LH y T4: 150 µg/ml FSH + 50 µg/ml LH. Resultados. No se presentó efecto (p>0.05) de los tratamientos suplementados con FSH y LH sobre la tasa de maduración; por el contrario, T1 fue diferente (p0.05) cuando el medio de maduración fue suplementado con FSH y LH. Conclusiones. La adición de LH en mayor proporción con respecto a la FSH en el medio de maduración induce una mayor tasa de maduración, fecundación y desarrollo embrionario in vitro a partir de oocitos bovinos recuperados postmortem.

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Referencias

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