Efficacy of essence oil supplementation to feeds on volatile fatty acid production

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Ahmet Tekeli Gültekin Yıldız Winfried Drochner Herbert Steingass



Objective. Determine the effect of some plant extract supplementation to Total Mixed Ration (TMR), concentrate and hay on volatile fatty acid (VFA) production at 8 and 24 hours (h) using in vitro gas production technique in cattle. Material and methods. Three fistulated Holstein dairy cows were used for rumen fluid collection for application of in vitro gas production technique. Four essence oils (T. vulgaris, O. vulgare, S. aromaticum, Z. officinale) were used as plant extracts. Results. Essence oil supplementations to the examined feed groups had significant effect only on C2/C3 VFA level at 8 h in all feed groups (p<0.05). C2/C3 VFA level at 8 h significantly increased in the groups with Oregano 25 ppm supplementation for TMR and concentrate and in the groups with Thymol 25 ppm supplementation for hay. C3 VFA level at 8 h significantly increased in the group that received Syzygium 200 ppm supplementation for hay. Different plant extracts supplemented to TMR, concentrate and hay significantly affected C2, C3, IC4, IC5, C5 and C2/C3 VFA levels at 24 h (p<0.05). Conclusions. The findings of the study indicate that moderate doses of plant extracts result in increased VFA levels in ruminants while higher doses demonstrate the opposite effect.

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1. Calsamiglia S, Busquet M, Cardoza PW, Castillejos L, Ferret A. Invited Review: Essential oils as modifiers of rumen microbial fermentation. J Dairy Sci 2007; 90:2580-2595. http://dx.doi.org/10.3168/jds.2006-644

2. Goel N, Sirohi SK, Dwivedi J. Studies on the effects of methanolic extract of Cinnamomum zeylanicum on in vitro methane inhibition and rumen fermentation patterns. J Chem Pharm Res 2011; 3(6):609-615.

3. Tekeli, A. Etlik civciv rasyonlarιnda doğal büyüme uyarιcι olarak bitkisel ekstraktlarιn ve propolisin kullanιm olanaklarι. [PhD Thesis]. Adana, Turkey: University of Çukuorova, Institute of Natural and Applied Sciences;2007.

4. Hart KJ, Yanez-Ruiz DR, Duval SM, McEwan NR, Newbold CJ. Plant extracts to manipulate rumen fermmentation. Anim Feed Sci Tech 2008; 147:8-35. http://dx.doi.org/10.1016/j.anifeedsci.2007.09.007

5. Hirasa K, Takemassa M. Spice science technology. Marcel Dekker Inc., New York, USA; 1998.

6. Botsoglou NA, Christaki E, Florou-Paneri P, Giannenas I, Papageorgiou G, Spais AB. The Effect of a mixture of herbal essential oils or α-Tocoheryl Acetate on performance parameters and oxidation of body lipid in broilers. S Afr J Anim Sci 2004; 34:52-61. http://dx.doi.org/10.4314/sajas.v34i1.4039

7. Jantan IB, Yassin MSM, Chin CB, Chen LL, Sim NL. Antifungal activity of the essential oils nine Zingiberaceae species. Brit Poultry Sci 2003; 41(5):392-397. http://dx.doi.org/10.1076/phbi.41.5.392.15941

8. Mitsch P, Zitterl-Eglseer K, Kohler B, Gabler C, Losa R, Zimpernik I. The effect of two different blends of essetial oil components on the proliferation of Clostridium perfringens in the intestines of broilers chickens. Poult Sci 2004; 83:669-675. http://dx.doi.org/10.1093/ps/83.4.669

9. Geissler Ch, Hoffmann M, Hickeli B. Determination of volatile fatty acids by gas chromatography (in German). Arch Anim Nutr 1976; 26:123-129.

10. Hinkelmann K, Kempthorne O. Design and analysis of experiments. Volume1, John Willey & Sons Inc., New Jersey, USA; 2008.

11. SPSS. Statistical Package for the Social Sciences [Sotfware] Version 22.0. SPSS Inc; 2013

12. Alataş MS, Umucalιlar HD. Bacteria of the rumen ecosystem and their roles. Atatürk University J Vet Sci 2011; 6(1):71-83.

13. Yang MG, Manoharan K, Mickelsen O. Nutritional contribution of volatile fatty acids from the cecum of rats. J Nutr 1970; 100:545-550.

14. Benchaar C, Chaves AV, Fraser GR, Wang Y, Beauchemin KA, McAllister TA. Effects of essential oils and their components on in vitro rumen microbial fermentation. Can J Anim Sci 2007; 413-419. http://dx.doi.org/10.4141/CJAS07012

15. Beauchemin KA, McGinn SM. Methane emissions from beef cattle: effects of fumaric acid, essential oil and canola oil. J Anim Sci 2006; 84:1489-1496. http://dx.doi.org/10.2527/2006.8461489x

16. Benchaar C, Petit HV, Berthiaume R, Whyte TD, Chouinard PY. Effects of addition of essential oils and monensin premix on digestion, ruminal fermentation, milk production, and milk composition in dairy cows. J Dairy Sci 2006; 89:4352-4364. http://dx.doi.org/10.3168/jds.S0022-0302(06)72482-1

17. Chaves AV, Stanford K, Gibson LL, McAllister TA, Benchaar C. Effects of carvacrol and cinnamaldehyde on intake, rumen fermentation, growth performance, and carcass characteristics of growing lambs. Anim Feed Sci Tech 2008; 145:396-408. http://dx.doi.org/10.1016/j.anifeedsci.2007.04.016

18. Kamalak A, Canbolat Ö, Özkan ÇÖ, Atalay A. Effect of thymol on in vitro gas production, digestibility and metabolizable energy content of alfalfa hay. Kafkas Univ J Fac Med 2011; 17(2):211-216.

19. Öztürk H, Demirtaş A, Salgιrlι Y, Pekcan M, Emre B, Fidancι UR. Effects of olive leaf extract on rumen microbial fermentation in in vitro semi-continuous culture system (RUSITEC). Ankara Üniv Vet Fak Derg 2012; 59(1):17-21. http://dx.doi.org/10.1501/Vetfak_0000002495

20. Castillejos L, Calsamiglia S, Ferret A. Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J Dairy Sci 2006; 89:2649-2658. http://dx.doi.org/10.3168/jds.S0022-0302(06)72341-4

21. Garcia V, Catala-Gregori P, Madrid J, Hernandez F, Megias MD, Andrade-Montemayor HM. Potential of carvacrol to modify in vitro rumen fermentation as compared with Monensin. Animal 2007; 1:675-680. http://dx.doi.org/10.1017/S1751731107730781

22. Chaves AV, Schei I, Wang Y, McAllister TA, Benchaar C. Effects of carvacrol and cinnamaldehyde on microbial fermentation when added to a barley-or corn-based diet in a continuous-culture system. Can J Anim Sci 2009; 89:97-104. http://dx.doi.org/10.4141/CJAS08062

23. Sirohi SK, Chaudhary PP, Goel N. Effect of inclusion of Myristica fragrans on methane production, rumen fermentation parameters and methanogens population. Vet World 2012; 5(6):335-340. http://dx.doi.org/10.5455/vetworld.2012.335-340

24. Cardozo PW, Calsamiglia S, Ferret A, Kamel C. Screening for the effects of natural plant extracts at different pH on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle. J Anim Sci 2005; 83:2572-2579. http://dx.doi.org/10.2527/2005.83112572x

25. Busquet M, Calsamiglia S, Ferret A, Kamel C. Plant extracts affect in vitro rumen microbial fermentation. J Dairy Sci 2006; 89:761-771. http://dx.doi.org/10.3168/jds.S0022-0302(06)72137-3

26. Petersen J, Lodge-Ivey SL, Browne-Silya J, Horvath MB. Extract from Larrea influences rumen fermentation. Proceedings, Western Section, American Society Anim Sci (ASAS) 2008; 59:367-370.

27. Canbolat O, Kalkan H, Karaman S, Filya I. The effect of essential oils on the digestibility, rumen fermentation and microbial protein production. Kafkas Univ J Fac Med 2011; 17(4):557-565.

28. Demirtaş A, Öztürk H, Pişkin I, Demirkιran D, Salgιrlι Y, Fidancι UR, Emre B. Effects of Rosemary and Sage extracts on ruminal fermentation using the rumen simulation technique (Rusitec). J Fac Vet Med Istanbul Univ 2011; 37(2):127-134.

29. Bodas R, López S, Fernández M, García-González R, Wallace RJ, González JS. Phytogenic additives to decrease in vitro ruminal methanogenesis. Nutritional and Foraging Ecology of Sheep and Goats. Ciheam Options Méditerranéennes 2009; 85:279-283.


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