Effect of different levels of L-carnitine and lysine-methionine on broiler blood parameters

Contenido principal del artículo

Autores

Babak Hosseintabar Mohammad Dadashbeiki Mehrdad Bouyeh Alireza Seidavi René van den Hoven Sandra Gamboa

Resumen

ABSTRACT

Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler.

RESUMEN

Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.

Palabras clave:

Detalles del artículo

Referencias

1. Hamerschlag K. Meat Eaters Guide to Climate Change + Health. Environmental Working Group. Meat Eaters Guide: Report 2011. [cited 2014 Mai 22] Avaible from:http://static.ewg.org/reports/2011/meateaters/pdf/report_ewg_meat_eaters_guide_to_health_and_climate_2011.pdf

2. Petracci M, Mudalal S, Bonfiglio A, Cavani C.Occurrence of white striping under commercial conditions and its impact on breast meat quality in broiler chickens. Poult Sci 2013; 92(6):1670-1675.
http://dx.doi.org/10.3382/ps.2012-03001

3. Cave MC, Hurt RT, Frazier TH, Matheson PJ, Garrison RN, McClain CJ et al. Obesity, inflammation, and the potential application of pharmaconutrition. Nutr Clin Pract 2008;23:16-34. http://dx.doi.org/10.1177/011542650802300116

4. Xu ZR, Wang MQ. Effects of L-carnitine on growth performance, carcass composition, and metabolism of lipids in male broiler. Poult Sci 2003;82:408-413. http://dx.doi.org/10.1093/ps/82.3.408

5. Kidd MT, Gilbert J, Corzo A, Page C, Virden WS, Woodworth JC. Dietary L-carnitine influences broiler thigh yield. Asian-Aust J Anim Sci 2009; 22(5):681-685. http://dx.doi.org/10.5713/ajas.2009.60665

6. Bouyeh M, Gevorgyan O. Influence of excess lysine and methionine on cholesterol fat and performance of broiler chicks. J Anim Vet Adv 2011; 10(12):1546-1550. http://dx.doi.org/10.3923/javaa.2011.1546.1550

7. Celik L, Ozturkcan O, InalL TC, Canacankatan N, Kayrin L. Effects of L-carnitine and niacin supplied by drinking water on fattening performance, carcass quality and plasma L-carnitine concentration of broiler chicks. Arch Anim Nutr 2003;57(2):127-136. http://dx.doi.org/10.1080/0003942031000107325

8. Mukhtar MA, Mohammed KA, Musa MH.Replacement value of lysine and methionine for super concentrate in broiler chick's yield and quality. J Sc Tech 2010; 11(2):27-29.

9. Si J, Kersey JH, Fritts CA, Waldroup PW. An evaluation of the interaction of lysine and methionine in diets for growing broilers. Int J Poult Sci 2004; 3(1):51-60. http://dx.doi.org/10.3923/ijps.2004.51.60

10. Corduk M, Ceylan N, Ildiz F. Effects of dietary energy density and L-carnitine supplementation on growth performance, carcass traits and blood parameters of broiler chickens. South African J Anim Sci 2007; 37(2):65-73. http://dx.doi.org/10.4314/sajas.v37i2.4029

11. Parizadian B, Ahangari YJ, Shargh MS, Sardarzadeh A. Effects of Different levels of L-carnitine supplementation on egg quality and blood parameters of laying japanese quail. Intern J Poult Sci 2011; 10(8):621-625. http://dx.doi.org/10.3923/ijps.2011.621.625

12. Arslan C. L-carnitine and its use as a feed additive in poultry feeding a review. Revue Méd Vét2006; 157(3):134-142.

13. Al-Mayah AAS. Immune response of broiler chicks to DL-methionine supplementation at different ages. Int J Poult Sci 2006; 5:169-172. http://dx.doi.org/10.3923/ijps.2006.169.172

14. Taraz Z, Dastar A. Effect of L-carnitine supplementing diets with different levels of protein on performance and blood parameters in broiler chickens. J Agric Sci Nat Res2008; 15(5):114-122.

15. Etim NAN, Akpabio U, Okpongete RO, Offiong EEA. Do Diets Affect Haematological Parameters of Poultry? British J Appl Sci & Techn 2014; 4(13):1952-1965. http://dx.doi.org/10.9734/BJAST/2014/8900

16. National Research Council (NRC). Nutrient Requirements of Poultry: Ninth Revised Edition. Washington, DC: The National Academies Press, 1994.

17. SAS, 2000. Statistical Analysis System. SAS User Guide: Release 9.2. SAS Institute INC, Cary NC, USA, 2000.

18. Donsbough AL, Powell S, Waguespack A, Bidner TD, Southern LL. Uric acid, urea, and ammonia concentrations in serum and uric acid concentration in excreta as indicators of amino acid utilization in diets for broilers. Poult Sci 2010; 89:287-94. http://dx.doi.org/10.3382/ps.2009-00401

19. Xie M, Hou SS, Huang W, Zhao L, Yu JY, Li WY et al. Interrelationship between methionine and cysteine of early peking ducklings. Poult Sci 2004; 83:1703-1708. http://dx.doi.org/10.1093/ps/83.10.1703

20. Çakir S, Yalçin S Effects of L-carnitine supplementation in diets with low or normal energy level on growth performance and carcass traits in broilers. Revue Méd Vét 2007; 158(6):291-296.

21. Chattopadhyay K, Mondal MK, Roy B. Comparative efficacy of DL-methionine and herbal methionine on performance of broiler chicken. Int J Poult Sci 2006; 5:1034-1039. http://dx.doi.org/10.3923/ijps.2006.1034.1039

22. Arslan C, Citil M, Saatci M. Effects of L-carnitine administration on growth performance, carcass traits, serum lipids and abdominal fatty acid compositions of geese. Rev Med Vet 2004: 155(6):315-320.

23. Murali P, George SK, Mercy AD, Dipu MT, Narayanankutty K. Effects of L-carnitine supplementation on serum lipid profile of broiler chicken fed with animal fat. Int J Agric and Food Sci Technol 2013; 4(8):773-774.

24. Adabi ShG, Moghaddam Gh, Taghizadeh A, Nematollahi A, Farahvash T. Effect of L-carnitine and vegetable fat on broiler breeder fertility, hatchability, egg yolk and serum cholesterol and triglyceride. Int Poult Sci 2006; 5(10):970-974. http://dx.doi.org/10.3923/ijps.2006.970.974

Descargas

La descarga de datos todavía no está disponible.