Contenido principal del artículo
AutoresJuliana Arango G Julián Echeverri Z Albeiro López H
Objective. To determine the association of intron 3 polymorphism of the bovine growth hormone (BGH) gene, with age at first service, first birth, first postpartum service and second birth in a population of Holstein cows in the state of Antioquia. Materials and methods. The study was conducted on 408 Holstein cows in 8 herds. Genotyping was performed using PCR-RFLP technique with DNA extracted from peripheral blood by the salting out technique. The Phenotypic information used was collected for 4 years, from a dairy production control program. To determine the association between characteristics and gene polymorphism, parametric statistical analyzes were performed as generalized linear models and linear regression analysis. Results. Allele frequency (positive) was 0.91 and was 0.09 for (negative) allele. Genotypic frequencies were 0.77, 0.2 and 0.03 for (positive/positive), (positive/negative) and (negative/negative) respectively. There were significant differences between the mean of age at first service, age at first birth, age at first postpartum service and age at second birth. For all of the (negative/negative) genotype characteristics there were greater ages for each event. Conclusions. These results suggest that intron-3 polymorphism of the BGH gene is associated with reproductive traits, facilitating the selection of individuals with favorable genotypes for use in breeding programs.
Detalles del artículo
2. Zhou G, Jin H, Liu C, Guo S, Zhu Q, Wu Y. Association of genetic polymorphism in GH gene with milk production traits in Beijing Holstein cows. J Biosci 2005; 30(5):595-598. http://dx.doi.org/10.1007/BF02703558
3. Abolfazl G, Rasoul T, Mortaza B, Cyrus A. Restriction fragment length polymorphism of bovine growth hormone gene intron 3 and its association with testis biometry traits in Iranian Holstein bull. Afr J Microbiol Res 2009;3(11):809-814.
4. Kanoth K, Kouno S, Okazaki A, Suzuki K, Obara Y. Interaction of GH polymorphism with body weight and endocrine functions in Japanese black calves. Domest Anim Endocrinol 2008;34(1):25-30. http://dx.doi.org/10.1016/j.domaniend.2006.10.003
5. Pereira A, Maurício M, Henrique N, Luciana C. Association of GH and IGF-1 polymorphisms with growth traits in a synthetic beef cattle breed. Genet Mol Biol 2005; 28(2):230-236. http://dx.doi.org/10.1590/S1415-47572005000200009
6. Curi A, Oliveira N, Silveira C, Lopes R. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest Prod Sci 2005; 94(3):159-167. http://dx.doi.org/10.1016/j.livprodsci.2004.10.009
7. Kanoth K, Kouno S, Okazaki A, Suzuki K, Obara Y. Interaction of GH polymorphism with body weight and endocrine functions in Japanese black calves. Domest Anim Endocrinol 2008; 34(1):25-30. http://dx.doi.org/10.1016/j.domaniend.2006.10.003
8. An P, Hou J, Wang l, Li G, Wang J , Song Y. Polymorphisms of the growth hormone gene and their effect on growth traits in Chinese goats. Meat Sci 2010; 86(3):758–763. http://dx.doi.org/10.1016/j.meatsci.2010.06.018
9. Patton J, Kenny D, Mcnamara S, Mee J, O'mara F, Diskin M, et al. Relationships among milk production, energy balance, plasma analytes, and reproduction in Holstein-Friesian cows. J Dairy Sci 2007; 90(2):649-658. http://dx.doi.org/10.3168/jds.S0022-0302(07)71547-3
10. Lucy M. Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reprod Domest Anim 2008; 43(2):31-39. http://dx.doi.org/10.1111/j.1439-0531.2008.01140.x
11. Rodríguez N, López A, Echeverri J. Estructura genética poblacional del gen lactoferrina bovino en vacas Holstein del departamento de Antioquia. Rev MVZ Córdoba 2013; 18(1):3355-3361.
12. Dybus A. Associations of growth (GH) and Prolactin (PRL) genes polymorphisms with milk production traits in Polish Black and White cattle. Anim Sci P 2002; 20(4):203-212.
13. Hartl DL, and Clark AG. Principles of population genetics. 4th edition Sunderland (MA): Sinauer Associates Inc Publishers 2007.
14. SAS 9.2 SQL. Statistical Analysis Systems Institute., Inc., Cary, N.C, USA, 2009.
15. Gorbani A, Vaez T, Bonyadi M, Amirinia C. A MspI PCR-RFLP within bovin growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. Afr J Biotechnol 2009; 8(19);4811-4816.
16. Mullen P, Lynch C, Waters S, Howard D, O'boyle P, Kenny D, et al. Single nucleotide polymorphisms in the growth hormone and insulin-like growth factor-1 genes are associated with milk production, body condition score and fertility traits in dairy cows. Genet Mol Res 2011; 10(3):1819-1830.¡ http://dx.doi.org/10.4238/vol10-3gmr1173
17. Katalin K, József V, Attila Z, István G, László F. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Arch Tierz 2006; 49(3):236-249.
18. Orrego J, Alfredo D, Echavarría L. Vida productiva y principales causas de descartes de vacas Holstein en la cuenca de lima. Rev int vet Perú 2003; 14(1):68-73.
19. Haworth G, Tranter W, Chuck J, Cheng Z, Wathes V. Relatioships between age at first calving and first lactation milk yield, and lifetime productivity and longevity in dairy cows. Vet Rec 2008; 162(20):643-647. http://dx.doi.org/10.1136/vr.162.20.643
20. Marini P. Comportamiento de vacas de diferentes edades al primer parto. Arch Zootec 2004; 53(202):205-208.
21. Arauna P, Chakravarty A, Bhattacharya T, Joshi B, Arjava S. Detection of Polymorphism of Growth Hormone Gene for the Analysis of Relationship between Allele Type and Growth Traits in Karan Fries Cattle. J Anim Sci 2004; 17(10):1334–1337.