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Genetic diversity of Prochilodus lineatus stocks using in the stocking program of Tietê River, Brazil

Genetic diversity of Prochilodus lineatus stocks using in the stocking program of Tietê River, Brazil



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Lopera-Barrero, N., Povh, J., Fornari, D. C., Rodriguez-Rodriguez, M., Alves dos Santos, S. C., & Ribeiro, R. (2013). Genetic diversity of Prochilodus lineatus stocks using in the stocking program of Tietê River, Brazil. Revista MVZ Córdoba, 18(3), 3759-3766. https://doi.org/10.21897/rmvz.145

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PlumX
Nelson Lopera-Barrero
Jayme Povh
Darci C. Fornari
Maria Rodriguez-Rodriguez
Silvio C. Alves dos Santos
Ricardo Ribeiro

ABSTRACT

Objective. Assess the genetic diversity in four brood stocks and one juvenile stock of curimba Prochilodus lineatus in a Hydropower plant in São Paulo - Brazil, using the Tietê River stocking program. Materials and methods. Five RAPD primers were used to amplify the extracted DNA from 150 fin-clip samples. Results. Fifty-nine fragments were polymorphic, 52 had frequencies with significant differences (p<0.05), 45 had low frequencies, 54 were excluded, and two were fixed fragments. High values for polymorphic fragments (71.19% to 91.53%) and Shannon index (0.327 to 0.428) were observed. The genetic divergence values within each stock were greater than 50%. Most of the genetic variation was found within the groups through the AMOVA analysis, which was confirmed by the results of the identity and genetic distance. High ancestry levels (FST) among the groups value indicated high and moderate genetic differentiation. The estimates of number of migrants by generation (Nm) indicated low levels of gene flow. High and moderate genetic divergence between groups (0.58 to 0.83) was observed. Conclusions. The results indicate high variability within the stocks, and genetic differentiation among them. The fish stocks analyzed represent a large genetic base that will allow the fish technicians to release juveniles without genetic risks to wild populations present in the river. These genetic procedures may be used as models for other migratory species, including those threatened by extinction.


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  1. Agostinho AA, Pelicice FM, Gomes LC. Dams and the fish fauna of the Neotropical region: impacts and management related to diversity and fisheries. Braz J Biol 2008; 68:1119-1132. http://dx.doi.org/10.1590/S1519-69842008000500019
  2. Garcez R, Calcagnotto D, Almeida-Toledo LF. Population structure of the migratory fish Prochilodus lineatus (Characiformes) from rio Grande basin (Brazil), an area fragmented by dams. Aquat Conserv 2011; 21:268-275. http://dx.doi.org/10.1002/aqc.1176
  3. Lopera-Barrero NM, Ribeiro RP, Povh JA, Vargas Mendez LD, Poveda-Parra AR. Produção de organismos aquáticos: uma visão geral no Brasil e no mundo. Brasil: Rígel & Livros; 2011.
  4. Agostinho AA, Thomaz SM, Gomes LC. Conservation of the biodiversity of Brazil's inland waters. Conserv Biol 2005; 19:646-652. http://dx.doi.org/10.1111/j.1523-1739.2005.00701.x
  5. Lopera-Barrero NM. Conservation of Brycon orbignyanus natural populations and stocks for their reproductive, genetic, environmental sustainability: A model for species threatened with extinction. Cien Inv Agr 2009; 36:191-208. http://dx.doi.org/10.4067/S0718-16202009000200004
  6. Viana AP, Lucena Frédou F, Frédou T, Torres MF, Bordalo AO. Fish fauna as an indicator of environmental quality in an urbanized region of the Amazon estuary. J Fish Biol 2010; 76:467-486. http://dx.doi.org/10.1111/j.1095-8649.2009.02487.x
  7. Carolsfed J, Harvey B, Baer A, Ross C eds. Migratory fishes of South America: Biology, Social Importance and Conservation Status. Victoria: World Fisheries Trust; 2003.
  8. Britto SGC, Sirol RN. Transposição de peixes como forma de manejo: as escadas do Complexo Canoas, médio rio Paranapanema, bacia do Alto Rio Paraná. En: Nogueira MG, Henry R, Jorcin A eds. Ecologia de Reservatórios: Impactos Potenciais, Ações de Manejo e Sistemas em Cascata. São Carlos: Rima; 2006.
  9. Agostinho AA, Marques EE, Agostinho CS, Almeida DA, Oliveira RJ, Melo JRB. Fish ladder of Lajeado Dam: migrations on one-way routes?. Neotrop Ichthyol 2007a; 5:121-130. http://dx.doi.org/10.1590/S1679-62252007000200005
  10. Povh JA, Ribeiro RP, Lopera-Barrero NM, Jacometo CB, Vargas L, Gomes PC et al. Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil. Sci Agric 2011; 68:308-313. http://dx.doi.org/10.1590/S0103-90162011000300006
  11. Araki H, Schmid C. Is hatchery stocking a help or harm? Evidence, limitations and future directions in ecological and genetic surveys. Aquaculture 2010; 308:2-11. http://dx.doi.org/10.1016/j.aquaculture.2010.05.036
  12. Kitada S, Shishidou H, Sugaya T, Kitakado T, Hamasaki K, Kishino H. Genetic effect of long-term stock enhancement programs. Aquaculture 2009; 290:69-79. http://dx.doi.org/10.1016/j.aquaculture.2009.02.011
  13. Gomes PC, Ribeiro RP, Lopera-Barrero NM, Povh JA, Vargas L, Sirol RN. Genetic diversity of three stocks of piapara (Leporinus elongatus) using RAPD. Acta Sci Anim Sci 2008; 30:241-247.
  14. http://dx.doi.org/10.4025/actascianimsci.v30i2.4710
  15. Lopera-Barrero NM, Ribeiro RP, Sirol RN, Povh JA, Gomes PC, Streit Jr DP et al. Genetic characterization of Brycon orbignyanus stocks used in stocking programs. Rev MVZ Córdoba 2008; 13:1110-1119.
  16. Povh JA, Ribeiro RP, Lopera-Barrero NM, Gomes PC, Blanck DV, Vargas L et al. Monitoring of the genetic variability of pacu, Piaractus mesopotamicus, of the stock enhancement program of the Paranapanema River. Arq Bras Med Vet Zootec 2009; 61:1191-1195. http://dx.doi.org/10.1590/S0102-09352009000500023
  17. Lopera-Barrero NM, Povh JA, Ribeiro RP, Gomes PC, Jacometo CB, Lopes TS. Comparison of DNA extraction protocols of fish fin and larvae samples: modified salt (NaCl) extraction. Cien Inv Agr 2008; 35:65-74.
  18. Miller M. Mantel-Struct: a program for the detection of population structure via mantel tests. J Hered 1999; 90:258-259. http://dx.doi.org/10.1093/jhered/90.1.258
  19. Yeh FC, Boyle TYZ, Xiyan JM. POPGENE Version 131: Microsoft Window-based freeware for population genetic analysis. Alberta: University of Alberta and Center for International Forestry Research; 1999.
  20. Miller MP. Tools of population genetic analysis (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data. Utah, Utah State University; 1997.
  21. Excoffier L, Laval G, Schneider S. Arlequin Ver. 3.1: an integrated software package for population genetics data analysis. Evol Bioinform Online 2005; 1:47-50.
  22. Workman PL, Niswander JD. Population studies on southwestern Indian tribes. II. Local genetic differentiation in the Papago. Am J Hum Genet 1970; 22:24-49.
  23. Wright S. Evolution and genetics of populations. Chicago: University of Chicago Press; 1978.
  24. Liu ZJ, Cordes JF. DNA marker technologies and their applications in aquaculture genetics. Aquaculture 2004; 238:1-37. http://dx.doi.org/10.1016/j.aquaculture.2004.05.027
  25. Jacometo CB, Lopera-Barrero NM, Rodriguez-Rodriguez M del P, Gomes PC, Povh JA, Streit Jr DP et al. Genetic variability of tambaqui (Teleostei: Characidae) from different regions of Brazil. Pesq agropec bras 2010; 45:481-487. http://dx.doi.org/10.1590/S0100-204X2010000500007
  26. Moreira AA, Hilsdorf AWS, Silva JV, Souza VR. Genetic variability of two Nile tilapia strains by microsatellite markers. Pesq Agropec Bras 2007; 42:521-526. http://dx.doi.org/10.1590/S0100-204X2007000400010
  27. Fopp-Bayat D, Jankun M, Kuzminski H. Genetic characterization of Polish culture brook trout, Salvelinus fontinalis (Mitchill), based on microsatellite DNA analysis. Arch Pol Fish 2010; 18:93-99. http://dx.doi.org/10.2478/v10086-010-0011-2
  28. Borrel YJ, Carleos CE, Asturiano JF, Bernardo D, Vázquez E, Corral N et al. Use of microsatellites and a combinatorial optimization approach in the acquisition of gilthead seabream (Sparus aurata L.) broodstocks for hatcheries. Aquaculture 2007; 269:200-210. http://dx.doi.org/10.1016/j.aquaculture.2007.04.055
  29. Lopera-Barrero NM, Vargas L, Sirol RN, Ribeiro RP, Povh JA, Mangolin CA. Genetic characterization of Brycon orbignyanus using the semi-natural system. Arq Bras Med Vet Zootec 2010; 62:184-191. http://dx.doi.org/10.1590/S0102-09352010000100025
  30. Lopes TS, Ribeiro RP, Lopera-Barrero NM, Sirol RN, Povh JA, Gomes PC et al. Genetic characterization of curimba (Prochilodus lineatus) stocks used in stock enhancement programs. Rev Bras Saúde Prod An 2008; 9:652-661.
  31. Lopera-Barrero NM, Ribeiro RP, Vargas L, Povh JA, Gomes PC, Mangolin CA et al. Genetic characterization of Prochilodus lineatus (Valenciennes, 1836) stocks using in stocking programs: importance for the ichthyofauna conservation and ecosystem. Biosci J 2008; 24:86-93.

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