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Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans

Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans



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Salas-Leiva, J., Dupré M E., & Salas-Leiva, D. (2016). Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans. Revista MVZ Córdoba, 21(1), 5258-5264. https://doi.org/10.21897/rmvz.35

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PlumX
Joan Salas-Leiva
Enrique Dupré M
Dayana Salas-Leiva

ABSTRACT

Objective. The effect of cryopreservation on the proximate composition of microalgae Chaetoceros calcitrans was evaluated. Materials and methods. C. calcitrans was cultured and cryopreserved using 5% (v/v) dimethylsulfoxide as cryoprotectant. The freezing was controlled at a rate of 3°C/min, up to -60°C and then the microalgae were immersed in liquid nitrogen (-196°C). After storage in liquid nitrogen, microalgae were rapidly thawed out and subcultured. The percentage of proteins, lipids and carbohydrates was analyzed using absorption spectrophotometry and the organic matter was studied by gravimetric analysis. Results. There was no significant variation between the proximate composition of C. calcitrans cryopreserved and the controls (p>0.05). Conclusion. Our results show that, despite low cell recovery after the preservation of this organism at low temperatures, there is no apparent loss of nutritional characteristics caused by the storing process at low temperatures.

RESUMEN

Objetivo. En este estudio se evaluó el efecto de la técnica de criopreservación sobre la composición proximal de la microalga Chaetoceros calcitrans. Materiales y métodos. C. calcitrans fue cultivada y criopreservada empleando dimetilsulfóxido al 5% (v/v) como crioprotector. La congelación fue controlada a una tasa de 3°C/min, hasta llegar a -60°C y luego las microalgas fueron sumergidas en nitrógeno líquido (-196°C). Posteriormente, las microalgas fueron descongeladas de manera rápida y nuevamente cultivadas. Seguidamente se analizó la cantidad porcentual de proteínas, lípidos y carbohidratos, empleando espectrofotometría de absorción y la materia orgánica por gravimetría. Resultados. Los resultados indicaron que la composición proximal posterior a la criopreservación no evidencia diferencias significativas respecto a los controles no criopreservados (p>0.05). Conclusiones. Los resultados indican que pese a la baja viabilidad post-criopreservación de C. calcitrans, no hay pérdida aparente de la composición nutricional debida al almacenamiento a bajas temperaturas.


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  1. Bui T, Ross I, Jakob G, Hankamer B. Impact of procedural steps and cryopreservation agents in the cryopreservation of Chlorophyte microalgae. PloS one 2013; 8(11):e78668. http://dx.doi.org/10.1371/journal.pone.0078668
  2. Salas-Leiva J, Dupré E. Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods. Lat Am J Aquat Res 2011; 39(2):271-279. http://dx.doi.org/10.3856/vol39-issue2-fulltext-8
  3. Sánchez-Saavedra M, Nú-ez-Zarco E. Photosynthetic and Biochemical Effects of Cold Storage on Marine Benthic Diatoms of the Mexican Pacific Coast. J World Aquacult Soc 2012; 43(2):249-258. http://dx.doi.org/10.1111/j.1749-7345.2012.00553.x
  4. Miller M, Quek K, Staehler K, Nalder T, Packer M. Changes in oil content, lipid class and fatty acid composition of the microalga Chaetoceros calcitrans over different phases of batch culture. Aquac Res 2012; 45(10):1634-1647. http://dx.doi.org/10.1111/are.12107
  5. Anning T, MacIntyre H, Pratt S, Sammes P, Gibb S, Geider R. Photoacclimation in the marine diatom Skeletonema costatum. Limnol Oceanogr 2000; 45(8): 1807-1817. http://dx.doi.org/10.4319/lo.2000.45.8.1807
  6. Thiyagarajan V, Harder T, Qiu J, Qian P. Energy content at metamorphosis and growth rate of the early juvenile barnacle Balanus amphitrite. Mar Biol 2003; 143(3): 543-554. http://dx.doi.org/10.1007/s00227-003-1077-9
  7. Illman A, Scragg A, Shales S. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Tech 2000; 27(8):631-635. http://dx.doi.org/10.1016/S0141-0229(00)00266-0
  8. Costard G, Machado R, Barbarino E, Martino R, Lourenço S. Chemical composition of five marine microalgae that occur on the Brazilian coast. Int J Fish Aquaculture 2012; 4(9):191-201.
  9. Nakanishi K, Deuchi K, Kuwano K. Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage. J Appl Phycol 2012; 24(6):1381-1385. http://dx.doi.org/10.1007/s10811-012-9790-8
  10. Esquivel B, Lobina D, Sandoval F. The biochemical composition of two diatoms after different preservation techniques. Comp Biochem Physiol 1993; 105B (2):369–373. http://dx.doi.org/10.1016/0305-0491(93)90243-x
  11. Nú-ez-Zarco E, Sánchez-Saavedra M. Cold Storage of Six Marine Benthic Diatoms Native to the Mexico Pacific Coast. J World Aquacult Soc 2011; 42(4):530-538. http://dx.doi.org/10.1111/j.1749-7345.2011.00495.x
  12. Taylor R, Fletcher L. Cryopreservation of eukaryotic algae: a review of methodologies. J Appl Phycol 1999; 10:481-501. http://dx.doi.org/10.1023/A:1008094622412
  13. Brown M, Jeffrey S, Volkman J, Dunstan G. Nutritional properties of microalgae for mariculture. Aquaculture 1997; 151:315-331. http://dx.doi.org/10.1016/S0044-8486(96)01501-3
  14. Banerjee S, Hew W, Khatoon H, Shariff M, Yusoff F. Growth and proximate composition of tropical marine Chaetoceros calcitrans and Nannochloropsis oculata cultured outdoors and under laboratory conditions. Afr J Biotechnol 2011; 10(8):1375-1383.
  15. Johnston J, Benson E, Harding K. Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in ribes germplasm. Plant Physiol Bioch 2009; 47:123–131. http://dx.doi.org/10.1016/j.plaphy.2008.10.008

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