Circulation of leptospira strains in rural areas from Cienaga de Oro, Córdoba- Colombia

Circulación de cepas de Leptospira en zonas rurales de Ciénaga de Oro, Córdoba – Colombia.

Leptospirosis is a zoonosis of worldwide distribution, which is cause of economic losses in farm animals with public health implications. Objetive. was to determine the presence of L. interrogans sensu lato in urine of cattle and water sources in rural areas from Córdoba (Colombia). Materials and methods. 78 urine samples were collected from cows and 39 samples of water for bacteriological culture and determination of the 16S rRNA gene from bacterial L. interrogans sensu lato. Although a definitive isolation was not achieved, L. interrogans sensu lato was detected by PCR in three urine samples and in one sample of waste water. Results. Circulation of L. interrogans sensu lato was confirmed by means PCR. Conclusions. These molecular evidences suggested the possibility of circulation of L. interrogans sensu lato in cows and water sources from rural areas of Ciénaga de Oro.

1. Ayral FC, Bicout DJ, Pereira H, Artois M, Kodjo A. Distribution of Leptospira serogroups in cattle herds and dogs in France. Am J Trop Med Hyg. 2014; 91:756-759.
2. Adler B, Peña MA. Leptospira and leptospirosis. Vet Microbiol. 2010; 140:287-296.3.
3. Lehmann JS, Matthias MA, Vinetz JM, Fouts DE. Leptospiral pathogenomics. Pathogens. 2014; 14;3(2):280-308.
4. Salgado M, Otto B. Sandoval E, Reinhardt G, Boqvist S. A cross sectional observational study to estimate herd level risk factors for
5. Leptospira spp. serovars in small holder dairy cattle farms in southern Chile. BMC Vet Res. 2014; 10: 126.
6. Fearnley C, Wakeley PR, Gallego-Beltran J, Dalley C, Williamson S, Gaudie C, Woodward MJ. The development of a realtime PCR to detect pathogenic Leptospira species in kidney tissue. Res Vet Sci. 2008; 85(1):8-16.
7. Congreso Nacional de Colombia. 1989. Ley 84. Por la cual se adopta el Estatuto Nacional de Protección de los Animales y se crean unas contravenciones y se regula lo referente a su procedimiento y competencia. República de Colombia, gobierno nacional. Diario Oficial 39120 de diciembre 27 de 1989. Bogotá.
8. Hamond C, Martins G, Lilenbaum W, Pinna M, Medeiros A. Infection by Leptospira spp. in cattle in a tropical region, Rio de Janeiro, Brazil. Am J Trop Med Hyg. 2015; 92 (1):210.
9. Gamage C, Koizumi N, Perera AK, Muto M, Nwafor-Okoli C, Ranasinghe S, Kularatne SA, Rajapakse RP, Kanda K, Lee RB, Obayashi Y, Ohnishi M, Tamashiro H. Carrier status of leptospirosis among cattle in Sri Lanka: a zoonotic threat to public health. Transbound Emerg Dis. 2014; 61(1):91-96.
10. Calderón A, Rodríguez V, Mattar S, Arrieta G. Leptospirosis in pigs, dogs, rodents, humans, and water in an area of the Colombian tropics. Trop Anim Health Prod. 2014;46 (2):427-432.
11. Hochedez P, Escher M, Decoussy H, Pasgrimaud L, Martinez R, Rosine J, Théodose R, Bourhy P, Picardeau M, Olive C, Ledrans M, Cabie A. Outbreak of leptospirosis among canyoning participants, Martinique, 2011. Euro Surveill. 2013; 18(18):20472.
12. Wynwood SJ, Graham GC, Weier SL, Collet TA, Mckay DB, Craig SB. Leptospirosis from water sources. Pathog Glob Health. 2014; 108(7):334-338.
13. Andre-Fontaine G, Aviat F, Thorin C. Waterborne Leptospirosis: Survival and preservation of the virulence of pathogenic leptospira spp. in fresh water. Curr Microbiol. 2015; 71(1):136-42.
14. Leshem E, Segal G, Barnea A, Yitzhaki S, Ostfeld I, Pitlik S, chwartz E. Travel-related leptospirosis in Israel: a nationwide study.
15. Am J Trop Med Hyg. 2010; 82(3):459-463.
16. Lagi F, Corti T, Meli M, Pinto A, Bartoloni A. Leptospirosis acquired by tourists in Venice Italy. J. Travel Med. 2013; 20(2):128-130.
17. Garvey P, Connell J, O’flanagan D, Mckeown P. Leptospirosis in Ireland: annual incidence and exposures associated with infection, Epidemiol Infect. 2014; 142:847-855.