AFLPS technique standarization for arrow cane (Gynerium sagittatum Aubl.) germplasm characterization

Estandarización de la técnica de AFLPS para la caracterización de germoplasma de caña flecha (Gynerium sagittatum Aubl.)

The arrow cane (Gynerium sagittatum Aubl.) is the raw material used for typical craftsmanship in the Colombian Northen Coast. Knowing the genetic variability allows to design and develop breeding strategies and genetic plant resource conservation. The objective of the study was to develop an AFLPs protocol to evaluate the genetic variability among 25 arrow cane accessions planted at the Universidad de Córdoba collection. A total of 223 loci ranging from 43 (E-ACC/M-CAG) to 72 loci (E-ACA/M-CTA). Primer average loci was 55,75. Combination E-ACC/M-CAG generated the highest polymorphism (39,53%). Locus profiles were different for all individuals in the population indicating a good genome scrutiny for analyzing variability and genetic structure of the species.

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