Effect of bencilaminopurine on in vitro multiplication rate of Dioscorea alata in liquid medium

Efecto del bencilaminopurina en medio liquido sobre la tasa de multiplicación in vitro en Dioscorea alata

The yam (Dioscorea sp.) is a plant of economic and nutritious importance in tropical and subtropical rainforest; it is characterized to be climber that possesses tubers rich in carbohydrates and vitamins. This research was done in the plant biotech lab of the University of Córdoba (Montería - Colombia) in 2001, with the objective of determining the effect of four BAP dose (0, 0.1, 0.2, and 0.3 mg L ^-1) on the in vitro multiplication rate of yam in a liquid medium. The number of shoot leaves and roots for explant after 30 days in culture were evaluated using a complete randomized design with 8 replicates. The results showed no statistical differences among the treatments, with a rate of 2.5 shoot per subculture period very similar to the rates obtained in semi solid medium without hormones. However, it was noted a better physiologic development in the leaves and longitude of the shaft when using liquid medium without hormones. The results indicate that is possible to use liquid medium to in vitro multiply yam and lower the costs avoiding the use of the gelling agent.

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