Detection of Yam mild mosaic virus BY IC-RT-PCR in leafhoopers, Rhynchosia minima and Dioscorea rotundata

Detección del virus del mosaico suave del ñame mediante IC-RT-PCR en Cicadélidos, Rhynchosia minima y Dioscorea rotundata

In Colombia, yam (Dioscorea spp) is grown and is very important to be staple food for the inhabitants of the Caribbean Region. Viral diseases cause losses in production and are essential to identify the vectors involved in their dispersion. The aim of this study was to detect Yam mild mosaic virus (YMMV) in symptomatic samples of Dioscorea rotundata Poir, in weed Rhynchosia minima L. and adult leafhooppers associated with yam crops. Leaf samples of R. minima and D. rotundata cv. “Botón” and leafhoppers were collected. The YMMV was detected by IC-RT-PCR. The results showed that R. minima is a weed host of YMMV, in addition adults of Oncometopia sp, Mareja sp and Parathona cayennensis are able to acquire this virus and the presence of viruses in crops yam in the Colombian Caribbean region was confirmed.

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