In vitro induction and proliferation of sweet potato (Ipomoea batatas (L.) Lam.) cell tissues in 2,4-D supplied medium

Inducción y proliferación in vitro de tejidos celulares de batata (Ipomoea batatas (L.) Lam.) en medio con 2,4-D

With the aim to develop a regeneration protocol for sweet potato (Ipomoea batatas (L.) Lam.) plants via somatic embryogenesis, several explants (leaf blade, petiole and petioled leaf blade) were cultured on MS medium supplemented with (mg L-1) thiamine HCl (1,0), sucrose (30.000), myo-inositol (100) and five 2,4- D concentrations (0,0; 2,26; 4,52; 9,04 and 18,08 µM) to evaluate cell tissue induction. Tissue proliferation was promoted on semisolid MS and liquid MS3:1N media and treatments were distributed with a complete randomized design. The data showed that 2,4-D was necessary for tissue induction and petioled leaf blade produced the highest level of tissue induction (90%). Tissue growth was evident between six and seven weeks after establishment with yellowish compact structures arising from the explants. Analysis performed showed significant effects (Pr< 0,05) of 2,4-D on fresh mass tissue increase in both, liquid and semisolid media.

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