Standardization of protocol to evaluate peroxidase activity in tree tomato (solanum betaceum cav.)

Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.)

The aim of this research was to obtain an analysis protocol of the enzymatic activity of Peroxidase (POD) in tree tomato pulp (Solanum betaceum Cav.). Variables were evaluated in the extraction process as the percentage of pulp (PP), of 20 to 50% and the centrifugation time (CT) of 10 to 30 min, while in the measurement was varied extract volume (EV) of 30 to 90 L. It was used an experimental central composite design 23 more star points and center points with response surface methodology replicated twice for a total of 32 experimental units. For optimization, the highest enzyme activity is presented using a PP of 60%, a CT of 34 min and a VE of 110 µl.

1. Agüero, M., Ansorena, M., Roura, S. and Del Valle, C. 2008. Thermal inactivation of peroxidase during blanching of butternut squash. Food Science and Technology.4: 401 - 407.
2. Akyol,Ç., Alpas, H. and Bayindirli, A. 2006. Inactivation of peroxidase and lipoxygenase in carrots, green beans, and green peas by combination of high hydrostatic pressure and mild heat treatment. European Food Research and Technology. 224(2):171 -176.
3. Anthon, G. and Barrett, D. 2002. Kinetic parameters for the thermal inactivation of quality-related enzymes in carrots and potatoes. Journal of Agricultural and Food Chemistry. 50(14):4119 - 25.
4. Anthon, G., Sekine, Y., Watanabe, N. and Barrett, D. 2002. Thermal Inactivation of Pectin Methylesterase, Polygalacturonase, and Peroxidase in Tomato Juice. Journal of agricultural and food chemistry.50:6153-6159.
5. Association Of Official Analytical Chemist International (AOAC).1997. Official methods of analysis of AOAC International. 16th Edition. Gaithersburg. USA. 340 p.
6. Bahçeci, K., Serpen, A., Gökmen, V. and Acar, J.2005. Study of lipoxygenase and peroxidase as indicator enzymes in green beans: change of enzyme activity, ascorbic acid and chlorophylls during frozen storage. Journal of Food Engineering. 66(2):187-192.
7. Brito, B., Espín, S., Villacrés, E. and Vallant, F. 2008. Tomate de árbol: Características físicas y nutricionales de la fruta importantes en la investigación y desarrollo de pulpas y chips. Disponible en: http://www.iniap.gob.ec/sitio/index. php?option=com_sobi2&sobi2Task=so bi2Details&catid=2&sobi2Id=221&Item id=. p.2.; consulta: Marzo, 2013.
8. Ceni, G., Baldissera, E., Antunes, O., Oliveira, J., Dariva, C. and Oliveira, D. 2008. Oxidases from mate tea leaves (Ilex paraguariensis): extraction optimization and stability at low and high temperatures. Bioprocess and Biosystems Engineering. 31(6):541 - 550.
9. Chisari, M., Barbagallo, R. and Spagna, G. 2007. Characterization of polyphenol oxidase and peroxidase and influence on browning of cold stored strawberry fruit. Journal of Agricultural and Food Chemistry. 55(9):3469 - 76.
10. Civello, P., Martinez, G., Chaves, A. and Anon, M. 1995. Peroxidase from Strawberry Fruit (Fragaria ananassa Duch.): Partial Purification and Determination of Some Properties. Journal of Agricultural and Food Chemistry. 43(10):2596 - 2601.
11. Cruz, R., Vieira, M. And Silva, C. 2006. Effect of heat and thermosonication treatments on peroxidase inactivation kinetics in watercress (Nasturtium officinale). Journal of Food Engineering.72:8 - 15.
12. Doan, S. and Doan, M. 2004. Determination of kinetic properties of polyphenol oxidase from Thymus (Thymus logicaulis subsp. chaubardii var. chaubardii). Food Chemistry. 88:69-77.
13. Duarte, M., Whitaker, J., Rojo, A., Garcia, B. and Regalado, C. 2003. Isolation and thermal characterization of an acidic isoperoxidase from turnip roots. Journal of Agricultural and Food Chemistry. 51(17):5096 - 5102.
14. Durango, K., Muñoz, K., Ocampo, P. y Londoño, J. 2007. Caracterización preliminar del enzima polifenol oxidasa en frutas tropicales: implicaciones en su proceso de industrialización. Scientia Et Technica. 13(33):161-164.
15. Ercan, S. y Soysal, Ç. 2011. Effect of ultrasound and temperature on tomato peroxidase. Ultrasonics Sonochemistry. 18:689 - 695
16. Fang, L., Jiang, B. and Zhang, T. 2008. Effect of combined high pressure and thermal treatment on kiwifruit peroxidase. Food Chemistry. 109(4):802 - 807.
17. FAO, 2006. Ficha técnica del tomate de árbol (Cyphomandra betacea). Disponible en: http://www.fao.org/inpho_archive/ content/documents/vlibrary/AE620s/ Pfrescos/TOMATEDEARBOL.HTM. 1p.; consulta: Marzo, 2013.
18. Fortea, M., López, S., Serrano, A., Carreño, J. and Nuñez, E. 2009. Kinetic characterisation and thermal inactivation study of polyphenol oxidase and peroxidase from table grape (Crimson seedless). Food Chemistry. 113(4):1008 - 1014.
19. Franco, G. 1998. Industrialización del tomate de árbol. p. 3-7. En: Memorias II seminario Frutales de Clima Frio moderado. Manizales. Colombia.
20. Ganjloo, A., Rahman, R., Bakar, J., Osman, A. and Bimakr, M. 2009. Modelling the Kinetics of Peroxidase Inactivation and Colour Changes of Seedless Guava (Psidium guajava L.) during Thermal Treatments. World Applied Sciences Journal. 7(1):105 - 112.
21. González, E., De Ancos, B. and Cano, M. 2000. Partial characterization of peroxidase and polyphenol oxidase activities in blackberry fruits. Journal of Agricultural and Food Chemistry. 48(11):5459 - 64.
22. Hiner, A., Hernández, J., Rodríguez, J., Garcia, F., Brisset, N., Smith, A. and Arnao, M. 2002. Reactions of the class II peroxidases, lignin peroxidase and arthromyces ramosus peroxidase, with hydrogen peroxide. Catalase-like activity, compound III formation, and enzyme inactivation. The Journal of Biological Chemistry.277 (30): 26879- 26885.
23. INSTITUTO COLOMBIANO DE NORMAS T…CNICAS y CERTIFICACI”N (ICONTEC). 1997. Frutas frescas. Tomate de árbol. Especificaciones. NTC 4105. Bogotá. 15 p.
24. Jang, J. and Moon, K. 2011. Inhibiton of polyphenol oxidase and peroxidase activities on fresh cut Apple by simultaneous treatment of ultrasound and ascorbic acid. Food Chemistry. 124(2):444 -449.
25. Jaramillo, S. 2003. Monografía sobre Phytophthora infestans (Mont) de Bary. Monografía. Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Medellín, Colombia. 5p.
26. Lee, C., Pennesi, A. and Dickson, M. 1984. Characterization of the cauli flower peroxidase isoenzyme. Journal of Agricultural and Food Chemistry. 32(1):18 - 21.
27. Lemmens, L., Tibäck, E., Svelander, C., Smout, C., AhrnÈ, L., Langton, M. and Alminger, M. 2009. Thermal pretreatments of carrot pieces using different heating techniques: Effect on quality related aspects. Innovative Food Science y Emerging Technologies. 10(4):522 - 529.
28. Martinez, G., Civello, P., Chaves, A. and AÒÛn, M. 2001. Characterization of peroxidase-mediated chlorophyll bleaching in strawberry fruit. Phytochemistry. 58(3):379 -87.
29. Mdluli, K.2005. Partial purification and characterisation of polyphenol oxidase and peroxidase from marula fruit (subsp. Caffra). Food Chemistry. 92(2):311- 323.
30. Morales, E., Chandia, V. and Cisneros, L. 2006. Thermal inactivation kinetics of peroxidase and lipoxygenase from broccoli, green asparagus and carrots. Journal of Food Science. 67(1):146 - 154.
31. Onsa, G., Bin Saari, N., Selamat, J. and Bakar, J. 2004. Purification and characterization of membrane-bound peroxidases from Metroxylon sagu. Food Chemistry. 85(3):365 - 376.
32. Raimbault, A., Marie, P., Horry, J., Francois, M.; Romuald, K. and Soler, A. 2011. Polyphenol oxidase and peroxidase expression in four pineapple varieties (Ananas comosus L.) after a chilling injury. Journal of Agricultural and Food Chemistry. 59(1):342 - 348.
33. Rani, D. and Abraham, T. 2006. Kinetic study of a purified anionic peroxidase isolated from Eupatorium odoratum and its novel application as time temperature indicator for food materials. Journal of Food Engineering. 77(3):594 - 600.
34. Roe, Simon. 2001. Protein Purification Techniques. Second Edition. Oxford University Press. Oxford. 240 p.
35. Rudra, G., Shivhare, U. and Basu, S. 2008. Thermal inactivation kinetics of Peroxidase in mint leaves. Journal of Food Engineering. 85(1):147 - 153.
36. SECRETARIA DE AGRICULTURA Y MEDIO AMBIENTE DE NARIÑO. 2012. Consolidado agropecuario, San Juan de Pasto. 115p.
37. Schweiggert, U., Schieber, A. and Carle, R.2005 Inactivation of peroxidase, polyphenoloxidase, and lipoxygenase in paprika and chili powder after immediate thermal treatment of the plant material. Innovative Food Science y Emerging Technologies. 6(4): 403 - 411.
38. Soysal, Ç., Söylemez, Z. and Bozoglu, F. 2004. Effect of high hydrostatic pressure and temperature on carrot peroxidase inactivation.European Food Research and Technology. 218(2):152 - 156.
39. Tabares, C. y Velásquez, J. 2003. Estudio de la vida de anaquel del tomate de árbol (Cyphomandra betacea) osmodeshidratado empacado en atmósferas modificadas. Trabajo de Grado de Ingeniero Químico Universidad Nacional de Colombia. Colombia. Manizales. 114p.
40. Terefe, N., Yang, Y., Knoerzer, K., Buckow, R. and Versteeg, C. 2010. High pressure and thermal inactivation kinetics of polyphenol oxidase and peroxidase in strawberry puree.Innovative Food Science & Emerging Technologies. 11(1):52 - 60.
41. Thongsook, T. and Barrett, D. 2005. Purification and partial characterization of broccoli (Brassica oleracea Var. Italica) peroxidases.Journal of Agricultural and Food Chemistry. 53(8):3206 - 3214.
42. Tijskens, L., Rodis, P., Hertog, M., Waldron, K., Ingham, L., Proxenia, N. and Van Dijk, C. 1997. Activity of peroxidase during blanching of peaches, carrots and potatoes. Journal of Food Engineering. 34(4):355 - 370.
43. Zhao, B. and Chang, K. 2008. Evaluation of effects of soaking and precooking conditions on the quality of precooked dehydrated Pea, Lentil and chickpea products. Journal of Food Processing and Preservation. 32(3):517-532.
44. Zhong, K., Wu, J., Wang, Z., Chen, F., Liao, X., Hu, X. and Zhang, Z. 2007. Inactivation kinetics and secondary structural change of PEF-treated POD and PPO.Food Chemistry. 100(1):115 - 123.
45. Zhou, Y., Dahler, J., Underhill, S. and Wills, R. 2003. Enzymes associated with blackheart development in pineapple fruit. Food Chemistry. 80(4):565 - 572.