Microinjertación de naranja Valencia y lima Tahití.

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Autores

Isidro E. Suárez https://orcid.org/0000-0002-8926-2781 Cristian Álvarez Correa https://orcid.org/0000-0003-3377-5227 Claudia Lopez Diaz https://orcid.org/0000-0001-6961-3153

Resumen

Para determinar la viabilidad de microinjertación en plantas de naranja Valencia y lima Tahití como un método de propagación masiva de plantas, diferentes pasos del proceso de microinjertación fueron evaluados. Las semillas de mandarina Cleopatra fueron establecidas in vitro en medio semisólido MS (Murashige y Skoog) con, o sin, GA3, para evaluar su efecto en el porcentaje y el tiempo de germinación, las plantas de naranja Valencia y lima Tahití fueron tratadas con 1 mg l-1 BAP o podadas manualmente para evaluar el efecto sobre la producción de brotes vegetativos, y el efecto de tres posiciones del ápice meristemático en la superficie del patrón decapitado, en el centro, en un lado y en un corte inclinado, sobre el porcentaje de éxito fue evaluado. Las repeticiones de todos los experimentos fueron distribuidas con un diseño completamente al azar, los datos de brotes vegetativos fueron analizados con ANAVA y los promedios separados con la prueba de Tukey. Los resultados evidenciaron que el GA3 no afectó el porcentaje y tiempo de germinación y la poda incrementó estadísticamente el número de brotes vegetativos. Los porcentajes de éxito de microinjertos de naranja Valencia (28%) y lima Tahití (14%) son consistentes con los resultados de otros estudios para recuperar plantas libres de enfermedades; sin embargo, para propagación masiva, se recomienda en futuros estudios considerar la evaluación de condiciones in vitro como aumento en la concentración de sacarosa, adaptación in vitro de los ápices meristemáticos y adición de auxina, entre otros.

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