The anthracnose of the yam and management strategies: a review

La antracnosis del ñame y estrategias de manejo: una revisión

Yam (Dioscorea spp.) is an edible tuber of economic importance in tropical regions, and a staple for more than 155 million people. Yam crop yields can be affected by devastating diseases such as anthracnose (Colletotrichum spp.), which can cause up to 80% loss in endemic areas, if control measures are not implemented. The following article is aimed to review the research conducted on anthracnose in yam, its biology and epidemiology, symptomatology, etiology and different management strategies. Disease symptoms, biology and epidemiology of the causal agent, Colletotrichum gloeosporioides, are described. Finally, the different management strategies such as genetic, cultural, biological and chemical control are presented. Integration of control methods should be used for disease management, starting with selection of fields with low amounts of primary inoculum, planting resistant or tolerant genotypes and using healthy asexual vegetative material protected with fungicides.

1. Abang, M., Green, K., Wanyera, N. and Lloba, C. 2001. Characterization of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria. In: Akoranda MO, Ngeve JM, eds. Root Crops in the 21st Century. Proceedings of the 7th Triennial Symposium of the International Society for Tropical Root Crops–Africa Branch, Cotonou, Bénin. 613-615.
2. Abang, M., Winter, S., Green, K., Hoffmann, P., Mignouna, H. and Wolf, G. 2002. Molecular identification of Colletotrichum gloeosporioides causing yam anthracnose in Nigeria. Plant Pathology 51(1): 63-71.
3. Aduramigba, A., Asiedu, R., Odebode, A. and Owolade, O. 2012. Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphism (AFLP) markers. African Journal of Biotechnology, 11(33): 8189-8195.
4. Agrios, G. 2005. Plant pathology. Academic press (pp. 487-494)
5. Amusa, N., Adegbite, A., Muhammed, S. and Baiyewu, R. 2003. Yam diseases and its management in Nigeria. African Journal of Biotechnology 2, 497-502.
6. Andres, C., AdeOluwa, O. and Bhullar, G. 2017. “Yam (Dioscorea spp.),” in Encyclopedia of Applied Plant Sciences, Vol. 3. eds B. Thomas, B. G. Murray, and D. J. Murphy (Waltham, MA: Academic Press), 435–441.
7. Arce, C., Varela, I. y Torres, S. 2019. Inhibición del crecimiento micelial de hongos asociados a antracnosis en ñame (Dioscorea alata). Agronomía Mesoamericana: 381-393.
8. Arya, R., Sheela, M., Jeeva, M. and Abhilash, P. 2019. Identification of Host Plant Resistance to Anthracnose in Greater Yam (Dioscorea alata L.). Int. J. Curr. Microbiol. App. Sci, 8(8):1690-1696.
9. Ayisah, K., Banito, A. and Oupressawa, M. 2019. Screening of D. alata varieties grown in Togo for resistance to yam anthracnose caused by Colletotrichum gloeosporioides. Journal of Applied Biosciences, 138, 14082-14091.
10. Ayodele, M., Hughes, J. and Asiedu, R. 2006. Yam antracnose disease: field symptoms and laboratory diagnostics. 1-16 p. https://www.iita.org
11. Beltrán, J. 2000. Caracterización y diagnóstico del género Colletotrichum causante de la antracnosis en ñame y otros cultivos. In: Ñame: producción de semilla por biotecnología (eds) Guzmán M y Buitrago G. Universidad Nacional. Bogota, Colombia. (pp. 55-66)
12. Abang, M., Winter, S., Mignouna, H., Green, K. and Asiedu, R. 2003. Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease. African Journal of Biotechnology 2:486-496.
13. Brown, J. 2006. Surveys of variation in virulence and fungicide resistance and their application to disease control. In The epidemiology of plant diseases Springer, Dordrecht. (pp. 81-115).
14. Campo, R y Pérez, D. 2015. EFECTO DE LA DENSIDAD DE SIEMBRA Y LA FENOLOGÍA DEL ÑAME (Dioscorea spp.) SOBRE LA ANTRACNOSIS (Colletotrichum gloeosporioides). Fitopatología Colombiana 39(2):37-40
15. Campo, R. 2000. La antracnosis, enfermedad limitante del cultivo del ñame. In ñame: producción de semilla por biotecnología (eds) Guzmán M y Buitrago G. Universidad Nacional. Bogotá, Colombia p 67- 70.
16. Campo, R. 2011. Manejo integrado de la antracnosis (Colletotrichum spp.) en ñame (Dioscorea alata) mediante el uso de alternativas para reducir el inóculo primario, la dispersión y el establecimiento del patógeno. informe final de proyecto. Universidad de Córdoba, Co. Doi:10.13140/rg.2.1.2576.9846
17. Campo, R. y Luna, J. 1998. Evaluación de la antracnosis en ñame Dioscorea alata var. Diamante 22 en monocultivo e intercalado con la variedad concha de coco. Ascolfi Informa 24 (5): 26-27 p.
18. Cao, X., Xu, X., Che, H., West, J. and Luo, D. 2019. Characteristics and distribution of Colletotrichum species in coffee plantations in Hainan, China. Plant Pathology 68(6):1146-1156.
19. Castro, L., Saquero, M. y Herrera, J. 2003. Caracterización morfológica y patogénica de Colletotrichum sp. como agente causal de la antracnosis en ñame Dioscorea sp. Revista colombiana de Biotecnología (1): 24-35.
20. Da Silva, L., Moreno, H., Correia, H., Santana, M. and de Queiroz, M. 2020. Colletotrichum: Species complexes, lifestyle, and peculiarities of some sources of genetic variability. Applied microbiology and biotechnology, 104(5), 1891-1904.
21. De Silva, D., Crous, P., Ades, P., Hyde, K. y Taylor, P. 2017. Life styles of Colletotrichum species and implications for plant biosecurity. Fungal Biology Reviews, 31(3): 155-168.
22. Dentika, P., Ozier, H. and Penet, L. 2021. Weeds as Pathogen Hosts and Disease Risk for Crops in the Wake of a Reduced Use of Herbicides: Evidence from Yam (Dioscorea alata) Fields and Colletotrichum Pathogens in the Tropics. Journal of Fungi, 7(4):283.
23. Dos Santos, W., Bezerra, P., da Silva, A., Veloso, J., Câmara, M. and Doyle, V. 2020. Optimal markers for the identification of Colletotrichum species. Molecular Phylogenetics and Evolution 143: 106694.
24. FAO. 2019. Datos sobre alimentación y agricultura. http://www.fao.org/faostat/es/#data
25. Fox, R. and Narra, H. 2006. Plant disease diagnosis. In The epidemiology of plant diseases Springer, Dordrecht. (pp. 1-42).
26. Frézal, L., Jacqua, G. and Neema, C. 2018. Adaptation of a fungal pathogen to host quantitative resistance. Frontiers in plant science 9:1554.
27. Frossard, E., Aighewi, B., Aké, S., Barjolle, D., Baumann, P., Bernet, T., Dao, D., Diby, L. Floquet, A., Hgaza, V., Ilboudo L., Kiba, D., Mongbo, R., Nacro, H., Nicolay, G., Oka, E., Ouattara, Y., Pouya, N., Senanayake, R., Six, J. and Traoré, O. 2017. The Challenge of Improving Soil Fertility in Yam Cropping Systems of West Africa. Front. Plant Sci. 8:1953.Doi: 10.3389/fpls.2017.01953
28. Fuentes, S. 2015. Caracterización morfológica, patogénica y molecular de especies de Colletrotrichum asociados al follaje del cultivo de ñame (Dioscorea spp.). Tesis Maestro en Ciencias, Universidad de Puerto Rico, Mayagüez p 1-50.
29. Fukada, F., Kodama, S., Nishiuchi, T., Kajikawa, N. and Kubo, Y. 2019. Plant pathogenic fungi Colletotrichum and Magnaporthe share a common G1 phase monitoring strategy for proper appressorium development. New Phytologist, 222(4):1909-1923.
30. González, M. 2012. El Ñame (Dioscorea spp.). Características, usos y valor medicinal. Aspectos de importancia en el desarrollo de su cultivo. Cultivos Tropicales, 33(4): 05-15.
31. Green, K. and Simons, S. 1994. Quantitative methods for assessing the severity of antracnose on yam (Dioscorea alata). Trop. Sci. 34: 216-224.
32. Guyader, S. 2017. Resilience of Colletotrichum gloeosporioides strains infecting yams to dry period and elevated temperature stresses. In 53. Annual Meeting of the Caribbean Food Crops Society (CFCS) (p. np).
33. Gwa, V. and Ekefan, E. 2017. Fungal Organisms Isolated from Rotted White Yam (Dioscorea rotundata) Tubers and Antagonistic Potential of Trichoderma harzianum against Colletotrichum Species. Agri Res & Tech: Open Access J, 10(3): 555787.
34. Han, Y., Zeng, X., Xiang, F., Zhang, Q., Cong, G., Chen, F. and Gu, Y. 2018. Carbendazim sensitivity in populations of Colletotrichum gloeosporioides complex infecting strawberry and yams in Hubei Province of China. Journal of integrative agriculture 17(6): 1391-1400.
35. Islam, A., Schreinemachers, P. and Kumar, S. 2020. Farmers’ knowledge, perceptions and management of chili pepper anthracnose disease in Bangladesh. Crop Protection, 133, 105139.
36. Ita, E., Uyoh, E., Nakamura, I. and Ntui, V. 2020. Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds. South African Journal of Botany, 130, 123-129.
37. Jehani, M., Patel, P. and Chaudhary, A. 2019. Evaluation of bioagents against Colletotrichum capsici caused anthracnose disease of yam (Dioscorea alata L.). Journal of Pharmacognosy and Phytochemistry 8(3):4788-4790.
38. Jiménez, Y., Campo, R. and López, L. 2009. Caracterización morfológica de aislamientos de Colletotrichum spp. causantes de la antracnosis del ñame. Dioscorea alata L. Fitopatología Colombiana 33(1):1-6
39. Kolade, O., Oguntade, O. and Kumar, L. 2018. Screening for resistance to Yam Anthracnose Disease. https://africayam.org/download/screening-resistance-yam-anthracnose-disease/.
40. Lin, C., Wu, W., Liao, X., Liu, W., Miao, W. and Zheng, F. 2018. First Report of Leaf Anthracnose Caused by Colletotrichum alata on Water Yam (Dioscorea alata) in China. Plant Disease 102(1): 248.
41. Liu, F., Tang, G., Zheng, X., Li, Y., Sun, X., Qi, X. and Zhang, S. 2016. Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China. Scientific reports 6: 32761.
42. Marroquín, N., Rodríguez, S., Gutiérrez, Y. y Hurtado, G. 2016. Caracterización molecular de Colletotrichum gloeosporioides aislado de plantas de ñame de la Costa Atlántica Colombiana utilizando la técnica “DNA Amplification Fingerprinting (DAF)”. Revista Colombiana de Biotecnología 18(1): 95-103.
43. Mercado, A., Dangon, F. y Páez, A. 2019. Aislamientos endofíticos de Colletotrichum spp. a partir de hojas y ramas de mango (Mangifera indica L.) cultivar Azúcar en el municipio de Ciénaga, Magdalena, Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 43(166): 65-77.
44. Mignouna, H., Mank, R., Ellis, T., Van Den Bosch, N., Asiedu, R., Abang, M. and Peleman, J. 2002. A genetic linkage map of water yam (Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance. Theoretical and Applied Genetics 105(5):726-735.
45. Ministerio de Agricultura y Desarrollo Rural - MADR. 2020. Organización de Cadena Nacional del Ñame. Internet. p 1-5. https://www.minagricultura.gov.co
46. Moreira, R., Zielinski, E., Castellar, C., Bergamin, A and De Mio, L. 2021. Study of infection process of five species of Colletotrichum comparing symptoms of glomerella leaf spot and bitter rot in two apple cultivars. European Journal of Plant Pathology, 159(1): 37-53.
47. Nwadili, C., Augusto, J., Bhattacharjee, R., Atehnkeng, J. and López, A. 2017. Comparative reliability of screening parameters for antracnose resistance in water yam Dioscorea alata. Plant Disease 101:209-216.
48. Onyeka, T., Petro, D., Ano, G., Etienne, S. and Rubens, S. 2006. Resistance in water yam (Dioscorea alata) cultivars in the French West Indies to anthracnose disease based on tissue culture‐derived whole-plant assay. Plant pathology 55(5): 671-678
49. Osorio, C 1989. Control químico de la antracnosis del ñame causada por Colletotrichum gloeosporioides, Penz (No. Doc. 25497) CO-BAC, Bogotá). 1-4 p.
50. Palaniyandi, S., Yang, S., Cheng, J., Meng, L. and Suh, J. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp. MJM5763. Journal of applied microbiology 111(2): 443-455.
51. Penet, L., Cornet, D., Blazy, J., Alleyne, A., Barthe, E. and Bussière, F. 2016. Varietal dynamics and yam agro-diversity demonstrate complex trajectories intersecting farmers’ strategies, networks, and disease experience. Front. Plant Sci. 7:1962. Doi: 10.3389/fpls.2016.01962
52. Penet, L., Guyader, S., Pétro, D., Salles, M. and Bussière, F. 2014. Direct splash dispersal prevails over indirect and subsequent spread during rains in Colletotrichum gloeosporioides infecting yams. PLoS One, 9(12), e115757
53. Popoola, A., Adedibu, B. and Ganiyu, S. 2013. Rapid assessment of resistance of tissue-cultured water yam (Dioscorea alata) and white guinea yam (Dioscorea rotundata) to anthracnose (Colletotrichum gloeosporioides Penz.). Archives Of Phytopathology And Plant Protection, 46(6): 663-669.
54. Raj, M., Hegde, V., Jeeva, M., Senthil, M., Nath, V., Vidyadharan, P. and Archana, P. 2013. Molecular diagnosis of Colletotrichum gloeosporioides causing anthracnose/dieback disease in greater yam (Dioscorea alata L.). Archives of Phytopathology and Plant Protection46(8): 927-936.
55. Reina, Y. 2012. El Cultivo de ñame en el caribe colombiano. Documentos de Trabajo Sobre Economía Regional y Urbana. 168, 2-34
56. Ripoche, A., Jacqua, G., Bussière, F., Guyader, S. and Sierra, J. 2007. Survival of Colletotrichum gloeosporioides (causal agent of yam anthracnose) on yam residues decomposing in soil. Appl. Soil Ecol. 38: 270–278. Doi: 10.1016/j.apsoil.2007.10.015
57. Rojo, I., García, R., Sañudo, J., Leon, J. y Allende, R. 2017. Proceso de infección de antracnosis por colletotrichum truncatum en papaya maradol. Revista Brasileira de Fruticultura, 39(SPE).
58. Rosado, Y. 2016. Evaluación de fungicidas orgánicos y convencionales para el control de enfermedades follares en ñame (Dioscorea alata L.). Tesis Maestro en Ciencias, Universidad de Puerto Rico, Mayagüez p 68-83.
59. Salehi, B., Sener, B., Kilic, M., Sharifi, J., Naz, R., Yousaf, Z. and Santini, A. 2019. Dioscorea plants: a genus rich in vital nutra-pharmaceuticals-A review. Iranian Journal of Pharmaceutical Research: IJPR, 18(Suppl1), 68.
60. Siddiqui, M., Ali, Z., Chittiboyina, A. and Khan, I. 2018. Hepatoprotective Effect of Steroidal Glycosides From Dioscorea villosa on Hydrogen Peroxide-Induced Hepatotoxicity in HepG2 Cells. Frontiers in pharmacology 9. 797.
61. Soares, A., Sousa, C., Garrido, M., Perez, J. y Almeida, N. 2006. Actinomicetos do solo com atividade in vitro contra patógenos do inhame Curvularia eragrostides e Colletotrichum gloeosporioides. Brazilian Journal of Microbiology 37(4): 456-461.
62. Sun, W., Wang, B., Yang, J., Wang, W., Liu, A., Leng, L. and Chen, S. 2017. Weighted gene co-expression network analysis of the dioscin rich medicinal plant Dioscorea nipponica. Frontiers in plant science 8: 789.
63. Suwannarat, S., Steinkellner, S., Songkumarn, P. and Sangchote, S. 2017. Diversity of Colletotrichum spp. isolated from chili pepper fruit exhibiting symptoms of anthracnose in Thailand. Mycological Progress, 16(7): 677-686.
64. Sweetmore, A., Simons, S. and Kenward, M. 1994. Comparison of disease progress curves for yam anthracnose (Colletotrichum gloeosporioides). Plant Pathology, 43(1): 206-215.
65. Than, P., Prihastuti, H., Phoulivong, S., Taylor, P. and Hyde, K. 2008. Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B 9(10): 764-778.
66. Tugizimana, F., Djami, A., Fahrmann, J., Steenkamp, P., Piater, L. and Dubery, I. 2019. Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum. Scientific reports, 9(1): 3290.
67. Uddin, M., Shefat, S., Afroz, M. and Moon, N. 2018. Management of anthracnose disease of mango caused by Colletotrichum gloeosporioides: A review. Acta Scientific Agriculture, 2(10): 169-177.
68. Weir, B., Johnston, P. and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73, 115–180.
69. Wumbei, A., Bawa, J., Akudugu, M. and Spanoghe, P. 2019. Absence of Effects of Herbicides Use on Yam Rots: A Case Study in Wulensi, Ghana. Agriculture 9(5): 95.