Development of an in vitro propagation protocol for roble (Tabebuia rosea Bertol DC)

Desarrollo de un protocolo para propagación in vitro de roble (Tabebuia rosea Bertol DC)

A significant advance for oak (Tabebuia rosea) micropropagation has been developed in order to massively propagate clones. The effect of three sodium hypochlorite concentration (1%, 2% and 3%) at three times (5, 10 y 15 min) on surface disinfestations were evaluated using explants consisting of 2-3 cm axillary shoots established on ½MS with (in mg L^-1) mio inositol (100), sucrose (30,000), thiamine HCl (0.4) and TC agar (8,000) (Sigma Co.). Five BAP (0.00, 2.22, 4.44, 8.88, 17.76 µM) concentration were evaluated for shoot multiplication and ANA at 0.00, 1.35, 2.69, 4.03 y 5.37 µM were evaluated for shoot rooting, both experiments had 15 replicates for treatment distributed in a complete randomized design. In vitro multiplied and rooted shoots were transferred to ex vitro for acclimatization. Ten percent sodium hypochlorite at 10 minutes was the best surface disinfecting treatment. BAP at 17.76 µM induced the higher multiplication rate while rooting was better with 5.37 µM ANA. Only in vitro rooted shoots survived to ex vitro conditions at a low rate indicating the need for further investigation to improve ex vitro acclimatization.

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