In vitro clonal propagation of Eucalyptus pellita f. muell from plus trees.

Propagación clonal in vitro de Eucalyptus pellita F. Muell a partir de árboles plus

The eucalyptus tree (Eucalyptus pellita) is a forester source that represents an important opportunity for the reforestation development in the Colombian Orinoquia. For the clonal propagation of trees selected by their excellent characteristics, growth and quality of the wood is required to develop efficient multiplication strategies. The objective of the present work was to propagate plustrees of E. pellita F. Muell, by techniques of plant tissue culture in vitro. For the establishment phase, nodal segments obtained from epicormic buds of plus trees were used as source of explants. Under aseptic conditions explants were disinfected with 70% ethanol during one minute, followed by immersion in 1% sodium hypochlorite during 15 minutes. Explants were planted in Murashige and Skoog media, obtaining up to 70% of the cultures free of contamination. For proliferation, the effect of different BAP concentrations was evaluated. BAP at 0,25 and 0,5 mg L-1 yielded 8,83 and 7,88 shoots per explant in 30 days, respectively. An 80% rooting was achieved by adding1 mg L-1 of AIB to the culture media. For acclimatization plantlets were transplanted in plastic trays containing peat moss under automatic mist condition, and 100% of survival was obtained.

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