Abscisic acid and sucrose affect in vitro tuber formation in yam (Dioscorea rotundata Poir.) plants

Ácido abscísico y sacarosa afectan la formación in vitro de túberos en plantas de ñame (Dioscorea rotundata Poir.)

Yam tuber (Dioscorea rotundata Poir.) is a staple for many populations in the Colombian Atlantic Coastland and the Caribbean basin, and its demand has recently increased in the international market; however, conventional crops practices negatively affect the quantity and quality of production. In order to provide mechanisms for the production of high quality planting material for yam, the effect of three sucrose concentrations combined with four abscisic acid levels on in vitro growing of microtubers from nodal explants was evaluated. Treatments were distributed using a complete randomized design, and the analysis of variance and Duncan´s Multiple Range Test showed that plant growth variables as well as microtuber number were significantly affected byABA/sucrose levels. Sucrose dose of 60 g L-1 combined with 3.0 mg L-1 of ABA induced the highest number of microtubers, while plants cultured in a medium supplied with 90 g L-1 of sucrose developed more stems, leaves and roots.

1. Acedo, V. Arradaza C. and Atilano, C. 2007. Microtuber production in Dioscorea alata L. variety “VU-2” as affected by growth regulators, Journal of Root Crops, 33(2):88-96.
2. Anike, F. Konan, K. Olivier, K. and Dodo, H. 2012. Efficient shoot organogenesis in petioles of yam (Dioscorea spp), Plant Cell, Tissue and Organ Culture 111(3):303-313.
3. Badr, A. Angers, P. and Desjardins, Y. 2015. Comprehensive analysis of in vitro to ex vitro transition of tissue cultured potato plantlets grown with or without sucrose using metabolic profiling technique, Plant Cell Tissue and Organ Culture 122(2): 491-508.
4. Balogun, M. 2009. Microtubers in yam germplasm conservation and propagation: The status, the prospects and the constraints, Biotechnology and Molecular Biology Reviews, 4(1):1-10.
5. Bansal, A. Kumari, V. Taneja, D. Sayal, R. and Das, N. 2012. Molecular cloning and characterization of granule-bound starch synthase I (GBSSI) alleles from potato and sequence analysis for detection of cisregulatory motifs, Plant Cell Tissue and Organ Culture 109(2): 247-261.
6. Bruno, M. Beyer, P. and Al-Babili, S. 2015. The potato carotenoid cleavage of B-ionone ring-containing carotenes and nonepoxidated xanthophylls, Archives of Biochemistry and Biophysics 572:126-33.
7. Cabrera, M. Gómez, R. Espinosa, E. López, J. Medero, V. Basail, M. and Arletys, A. 2011. Yam (Dioscorea alata L.) microtuber formation in Temporary Immersion System as planting material, Biotecnología Aplicada 28: 268-271.
8. Cereda, M. 2002. Importancia, modo de consumo e perspectiva para raíces e tuberculos horticolas no Brasil, In: Carmo, C,A, Inhame e taro: sistema de produção familiar, Vitoria: Instituto Capixaba de pesquisa, asistencia Técnica e Extensao Rural, Brasil, p23-32.
9. Chen, F. Fu, Y. Wang, D. Gao, X. and Wang, L. 2007. The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino, Journal of Plant Growth Regulator 26(1):38-45.
10. Dai, Z. Meddar, M. Renaud, C. Merlin, I. Hilbert, G. Delrot, S. and Gomès, E. 2014. Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation, Journal of Experimental Botany 65(16):4665-4677.
11. Deroles, S. Seelye, J. Javellana, J. and Mullan, A. 2010. In vitro propagation of Sandersonia aurantiaca Hook using thidiazuron, Plant Cell Tissue and Organ Culture 102(1): 115-119.
12. Guo, L. Yu, L. Fan, Y. Lu, Neng, Yin, M. Zhang, F. and Yang, Q. 2010. Cloning and characterization of a potato TFL1 gene involved in tuberization regulation, Plant Cell Tissue and Organ Culture103(1): 103-109.
13. Hayashi, Y. Takahashi, K. Inoue, S. and Kinoshita, T. 2014. Abscisic acid suppresses hypocotyl elongation by dephosphorylating plasma membrane H(+)-ATPase in Arabidopsis thaliana, Plant Cell Physiology 55(4): 845-853.
14. Hu, Y. Li, Y. Zhang, J. Liu, H. Tian, M. and Huang, Y. 2012. Binding of ABI4 to a CACCG motif mediates the ABA-induced expression of the ZmSSI gene in maize (Zea mays L.) endospem, Journal of Experimental Botany 63(16): 5979-5989.
15. Jang-Ho. 2015. The laccase promoter of potato confers strong tuber-specific expression in transgenic plants, Plant Cell Tissue and Organ Culture 120(1):57-68.
16. Jean, M. and Cappodocia, M. 1991. In vitro tuberization in Dioscorea alata L. “Brazo fuerte” y “Florido” y D. abbyssinica. Hoch. Plant Cell Tissue and Organ Culture 26:147-152.
17. Karmoker, J. and Van Steveninck, R. 1979. Stimulation of volume flow and ion flux by abscisic acid in excised root systems of Phaseolus vulgaris L. CV Redland Pioneer, Physiologia Plantarum 141(1):37-43.
18. Lema-Ruminska, J. Goncerzewicz, K. and Gabriel, M. 2013. Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in Cactus Copiapoa tenuissima Ritt, Forma mostruosa, The Scientific World Journal 2013ID513985 7p.
19. López, O. 2015. Inducción de estructuras vegetativas de caña flecha (Gynerium sagitatum Aubl.) in vitro, Tesis Licenciado en Ciencias Naturales, Universidad de Córdoba, Montería, 40p.
20. McKoy, M. Thomas, P. Asemota, H. Omoruyi, F. and Simon, O. 2014. Effects of Jamaican bitter yam (Dioscorea polygonoides) and diosgenin on blood and fecal cholesterol in rats, Journal of Medicine Food 17(11): 1183.
21. Muñiz-Garcia, M. Stritzler, M. and Capiati, D. 2014. Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation, Planta 239(3): 615- 631.
22. Murashige, T. and Skoog, F. 1962. A revised medium for proper growth and bioassays with tobacco tissue culture, Physiologia Plantarum 15:473-497.
23. Ncube, B. Finnie, J. and Van, J. 2014. Carbonnitrogen ratio and in vitro assimilate partitioning patterns in Cyrtanthus guthrieae L. Plant Physiology and Biochemistry 74:246-254.
24. Ng, S. 1988. In vitro tuberization in white yam (Dioscorea rotundata Por.), Plant Cell Tissue and Organ Culture14:121-128.
25. Olivier, K. Honan, K. Anike, F. Agbo, G. and Dodo, H. 2012. In vitro induction of minitubers in yam (Dioscorea cayenensis – D. rotundata complex), Plant Cell Tissue and Organ Culture 109(1):179-189.
26. Ondo, P. Kevers, C. and Dommes, J. 2009. Effects of reducing sugar concentration on in vitro tuber formation and sprouting in yam (Dioscorea cayenensis – D. rotundata complex), Plant Cell Tissue and Organ Culture 99:55–59.
27. Perea, M. and Buitrago G. 2000. Aplicación de la Biotecnología Agrícola al cultivo del Ñame, En: producción de semillas por Biotecnología, Universidad Nacional de Colombia, Bogotá D.C. Colombia, p17- 19.
28. Prat, S. 2010. Hormonal and day length control of potato tuberisation, Plant Hormones, Madrid, p574-596.
29. Rayirath, U. Lada, R. Caldwell, C. Asiedu, S. and Sibley, K. 2011. Role of ethylene and jasmonic acid on rhizome induction and growth in rhubarb (Rheum rhabarbarum L.), Plant Cell Tissue and Organ Culture 105(2): 253-263.
30. Rêgo, T. Ash, L. Pessoa, L. Feijo, M. Leite, J. Dos santos Ade, S. Da Costa, C. and Boaventura, G. 2014. The intake of yam (Dioscorea bulbifera Linn.) attenuated the hyperglycemia and the bone fragility in female diabetic rats. Nutrition Hospital 29:370.
31. Rook, F. Hadingham, S. Li, Y. and Bevan, M. 2006. Sugar and ABA response pathways and the control of gene expression, Plant Cell and Environment 29:426–434.
32. Salazar, R. and Beltrán, J. 2003. Microtuberización en ñame (Dioscorea alata L.) var. “Pico de Botella”, Revista Colombiana de Biotecnología 4(2):27- 32.
33. Schussler, J. Brenner, M. and Brun, W. 1984. Abscisic Acid and Its Relationship to Seed Filling in Soybeans, American Society of Plant Biologists, Plant Physiology 76(2): 301-306.
34. Scott, G. Rosegrant, M. and Ringler, C. 2006. Roots and tubers for the 21st Century: Trends, projections, and policy options, Food Agriculture and the Environment Discussion 31, Washington, DC: International Food Policy Research Institute (IFPRI) and International Potato Center (CIP), Lima, p1-63.
35. Sharma, P. Pandey, A. Bhattacharya, A. Nagar, P. and Ahuja, P. 2004. ABA associated biochemical changes during somatic embryo development in Camellia sinensis (L.) O. Kuntze. Plant Physiology 161:1269-1276.
36. Suárez, I. Torres, L. and Litz R. 2011. Somatic embryogenesis in yam (Dioscorea rotundata), Revista Facultad Nacional de Agronomía Medellín 64(2):6037-6042.
37. Tamiru, M. Becker, H. and Maass, B. 2008. Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia, Genetic Resources Crop Evolution 55:115-131.
38. Wang, Z. Shen, J. Ludewing, U. and Neumann, G. 2015. A re-assessment of sucrose signaling involved in cluster-root formation and function in phosphatedeficient white lupin (Lupinus albus), Physiologia plantarum 154(3):407-419.
39. Yan, H. Yang, L. and Li, Y. 2011. Axilliary shoot proliferation and tuberization of Dioscorea fordii Prainet Burk. Plant Cell Tissue and Organ Culture 154(2): 193- 198.